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| Product Name | >=15 U/mg Sarcosine Oxidase for Enzymatic Creatinine Assays |
| Catalog No. | IEC-HMM-0256 |
| Description | Alta DiagnoTech sarcosine oxidase is a yellow amorphous lyophilized enzyme powder for hydrogen-peroxide-generating creatinine assay systems. |
| Intended Use | Enzyme raw material for research and bulk formulation of enzymatic creatinine reagents. |
| Performance Range / Specifications | Specific activity >=15 U/mg dry powder; protein purity >=90%; catalase <=0.03%; creatinine amidohydrolase <=0.01%; creatinase <=0.01%; ATPase <=0.005%. |
| Storage Conditions | Store at -25 to -15 degrees C |
| Shelf Life | Maintains at least 90% activity for 12 months at -25 to -15 degrees C |
| Package Specifications | 3 kU, 100 kU, 500 kU, 1,000 kU or custom packaging |
| Product Form | Yellow amorphous lyophilized powder |
| Purity | >=90% protein purity |
| Activity / Unit Definition | One unit is the amount of enzyme that generates 1 umol hydrogen peroxide per minute under the stated conditions. |
| Molecular Weight | 45 kDa by SDS-PAGE |
| Source / Origin | Microbial source |
| pH Range / Optimal pH | Optimum pH 8.0; stable at pH 7.0-9.5 for 16 hours at 25 degrees C |
| Appearance | Yellow amorphous lyophilized powder |
| Alias | Sarcosine Oxidase |
| Product Type | Sarcosine oxidase enzyme raw material |
| Function / Efficacy | Oxidize sarcosine and generate hydrogen peroxide for coupled creatinine measurement. |
| Abbreviation | SOX |
| EC Number | EC 1.5.3.1 |
| Specific Activity | >=15 U/mg dry powder |
| Isoelectric Point | 5.3 |
| Inhibitors | SDS, Zn2+ and Cd2+ |
| Optimal Temperature | 50 degrees C |
| Thermal Stability | Stable below 50 degrees C at pH 7.5 for 30 minutes |
| Michaelis Constant | 5.6 x 10^-3 M for sarcosine |
For research use only, not for clinical use.
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