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| Product Name | >=15 U/mg Malate Dehydrogenase for 340 nm Oxaloacetate/NADH Assays |
| Catalog No. | IEC-HMM-0232 |
| Description | Alta DiagnoTech malate dehydrogenase is a white amorphous enzyme powder characterized for AST and carbon-dioxide assay raw-material applications. |
| Intended Use | Enzyme raw material for aspartate aminotransferase and carbon-dioxide biochemical assay development. |
| Principle / Technology | Malate dehydrogenase converts oxaloacetate and NADH to L-malate and NAD+. |
| Detection Method | Kinetic absorbance assay at 340 nm |
| Performance Range / Specifications | Specific activity is at least 15.0 U/mg; stated activity-method linear range is 800-8,000 U/L. |
| Reaction Conditions / Protocol | At 30 degrees C, combine 0.840 mL potassium-phosphate buffer at pH 7.50, 0.030 mL oxaloacetate solution and 0.030 mL NADH solution; preincubate for 2 minutes, add 0.015 mL sample and monitor A340 from 15 to 55 seconds. The stated method factor is 9,807. |
| Storage Conditions | Store at -20 degrees C |
| Shelf Life | At least 1 year at -20 degrees C |
| Product Form | White amorphous powder |
| Activity / Unit Definition | One unit is the amount of enzyme that generates 1 umol NAD+ per minute under the stated conditions. |
| Molecular Weight | Approximately 40 kDa |
| pH Range / Optimal pH | Stable at pH 5.0-9.0; optimum pH approximately 6.0 |
| Recommended Buffer System | Sample diluent: Tris, L-aspartate, dipotassium EDTA and BSA at pH 7.50. Assay buffer: 0.1 M potassium phosphate at pH 7.50 with freshly prepared oxaloacetate and NADH solutions. |
| Appearance | White amorphous powder |
| Alias | Malate Dehydrogenase |
| Product Type | Malate dehydrogenase enzyme raw material |
| Function / Efficacy | Reduce oxaloacetate to L-malate while oxidizing NADH to NAD+. |
| Abbreviation | MDH |
| Customization | Specifications can be customized |
| Specific Activity | >=15.0 U/mg |
| Isoelectric Point | 5.6 |
| Inhibitors | Not inhibited by sodium azide |
| Optimal Temperature | 40 degrees C |
| Thermal Stability | Stable below 50 degrees C |
| Michaelis Constant | 4.7 x 10^-5 M for L-malate; 6.4 x 10^-5 M for oxaloacetate; 2.5 x 10^-5 M for NADH |
| Detection Wavelength | 340 nm |
| Linear Range | 800-8,000 U/L |
For research use only, not for clinical use.
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