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| Product Name | >=15 U/mg Cholesterol Esterase for TCHO, HDL-C and LDL-C Assays |
| Catalog No. | IEC-HMM-0163 |
| Description | Alta DiagnoTech cholesterol esterase is an off-white amorphous enzyme powder for total cholesterol, HDL-C and LDL-C biochemical reagent development. |
| Intended Use | Enzyme raw material for total cholesterol, HDL cholesterol and LDL cholesterol biochemical assay kits. |
| Detection Method | Cholesterol-oxidase/peroxidase-coupled absorbance assay at 505 nm |
| Performance Range / Specifications | Specific activity >=15 U/mg; assay linear range 100-300 U/L. |
| Reaction Conditions / Protocol | Use potassium phosphate reagent at pH 7.0 containing 4-aminoantipyrine, peroxidase and cholesterol oxidase, together with phenol and a cholesterol-linoleate/Triton X-100/sodium-cholate substrate. Combine 0.3 mL reagent 1, 0.45 mL phenol reagent and 0.45 mL substrate, preincubate for 180 seconds at 37 degrees C, add 0.04 mL sample and determine the one-minute rate after 2 minutes at 505 nm. |
| Storage Conditions | Store at -20 degrees C |
| Shelf Life | At least 1 year at -20 degrees C |
| Product Form | Off-white amorphous powder |
| Activity / Unit Definition | One unit produces 1 umol hydrogen peroxide, equivalent to 0.5 umol quinone dye, per minute under the stated conditions. |
| Molecular Weight | 52 kDa |
| pH Range / Optimal pH | Stable at pH 4.0-9.0; optimum pH 5.0-8.5 |
| Appearance | Off-white amorphous powder |
| Alias | Cholesterol esterase |
| Product Type | Cholesterol esterase enzyme raw material |
| Function / Efficacy | Hydrolyze cholesterol esters for coupled cholesterol measurement. |
| Abbreviation | COE |
| Customization | Specifications can be customized |
| Specific Activity | >=15 U/mg |
| Isoelectric Point | 5.5 |
| Inhibitors | Not inhibited by sodium azide |
| Optimal Temperature | 45 degrees C |
| Thermal Stability | Stable to 50 degrees C |
| Michaelis Constant | 1.9 x 10^-5 mol/L for cholesterol linoleate |
| Detection Wavelength | 505 nm |
| Linear Range | 100-300 U/L |
For research use only, not for clinical use.
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