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| Product Name | >=1.5 U/mg Acetyl-CoA Synthase for Coupled Free Fatty Acid Assays |
| Catalog No. | IEC-HMM-0140 |
| Description | Alta DiagnoTech acetyl-CoA synthase is a white amorphous enzyme powder for non-esterified fatty acid reagent development. |
| Intended Use | Enzyme raw material for non-esterified fatty acid biochemical assay kits. |
| Detection Method | Acetyl-CoA-oxidase/peroxidase-coupled endpoint assay at 500 nm |
| Performance Range / Specifications | Specific activity >=1.5 U/mg; stated assay range 100-200 U/L. |
| Reaction Conditions / Protocol | Use potassium phosphate at pH 7.5 with ATP, MgCl2, CoA and palmitic acid in Triton X-100. Incubate 320 uL cofactor reagent plus 80 uL substrate for 1 minute at 37 degrees C, add 20 uL sample and react for 10 minutes. Add 800 uL color reagent and 20 uL phenol, incubate for 2 minutes, add 20 uL acetyl-CoA oxidase, react for 5 minutes at 37 degrees C and read at 500 nm against a dilution-buffer blank. |
| Storage Conditions | Store at -20 degrees C |
| Shelf Life | At least 1 year at -20 degrees C |
| Product Form | White amorphous powder |
| Activity / Unit Definition | One unit converts 1 umol palmitic acid per minute under the stated assay conditions. |
| Molecular Weight | 64 kDa |
| pH Range / Optimal pH | Stable at pH 6.5-7.5; optimum pH 8.0 |
| Appearance | White amorphous powder |
| Alias | Acetyl-CoA synthase |
| Product Type | Acetyl-CoA synthase enzyme raw material |
| Function / Efficacy | Activate palmitic acid with CoA and ATP for coupled free-fatty-acid measurement. |
| Abbreviation | ACS |
| Customization | Specifications can be customized |
| Specific Activity | >=1.5 U/mg |
| Isoelectric Point | 5.2 |
| Inhibitors | Not inhibited by sodium azide |
| Optimal Temperature | 37 degrees C |
| Thermal Stability | Stable below 50 degrees C in the presence of 2 mM ATP |
| Michaelis Constant | 1.1 x 10^-5 M for palmitic acid; 1.7 x 10^-4 M for ATP; 3.2 x 10^-4 M for CoA |
| Detection Wavelength | 500 nm |
| Linear Range | 100-200 U/L |
For research use only, not for clinical use.
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