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| Product Name | >=13,000 U/mg Catalase for Rapid Hydrogen Peroxide Removal |
| Catalog No. | IEC-HMM-0161 |
| Description | Alta DiagnoTech catalase is a light-green to brown-green amorphous lyophilized enzyme powder that decomposes hydrogen peroxide to water and oxygen. |
| Intended Use | Removal of hydrogen peroxide from biochemical reaction systems, including creatinine-related reagent workflows. |
| Principle / Technology | Hydrogen peroxide consumption is measured spectrophotometrically at 240 nm. |
| Detection Method | Spectrophotometric hydrogen peroxide-consumption assay at 240 nm |
| Performance Range / Specifications | Specific activity >=13,000 U/mg dry powder; protein purity >=90%; peroxidase contamination <=0.02%; solution pH 7.0-8.0 when dissolved at 10 mg/mL in deionized water; 150 IU completely removes 3% hydrogen peroxide within 10 minutes. |
| Storage Conditions | Store at -25 to -15 degrees C |
| Package Specifications | 1 MU, 100 MU, 500 MU or 1,000 MU |
| Product Form | Light-green to brown-green amorphous lyophilized powder |
| Purity | >=90% protein purity |
| Activity / Unit Definition | One unit is the amount of enzyme that consumes 1 umol hydrogen peroxide per minute at 37 degrees C and pH 7.0. |
| Source / Origin | Microbial source |
| pH Range / Optimal pH | Optimum pH 6.0-9.0; stable at pH 4.5-10.0 for 16 hours at 25 degrees C |
| Recommended Buffer System | pH stability buffers: acetate at pH 4.5-5.5, phosphate at pH 6.0-8.0, Tris-HCl at pH 8.0-9.0 and glycine-NaOH at pH 9.0-10.0. Stability studies use 1 mg/mL enzyme. Temperature testing uses 50 mM potassium phosphate at pH 7.0. |
| Appearance | Light-green to brown-green amorphous lyophilized powder |
| Alias | Catalase |
| Product Type | Catalase enzyme raw material |
| Function / Efficacy | Decompose hydrogen peroxide to water and oxygen. |
| Abbreviation | CAT |
| EC Number | EC 1.11.1.6 |
| Specific Activity | >=13,000 U/mg dry powder |
| Optimal Temperature | 25-60 degrees C |
| Thermal Stability | Stable below 65 degrees C at pH 7.5 for 30 minutes |
| Detection Wavelength | 240 nm |
For research use only, not for clinical use.
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