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| Product Name | ≥1,300 U/mg Malate Dehydrogenase for AST Reagent Manufacturing |
| Catalog No. | IEC-HMM-0233 |
| Description | Alta DiagnoTech malate dehydrogenase is supplied as a white amorphous lyophilized powder for biochemical reagent formulation. |
| Intended Use | For research, development and large-scale preparation of aspartate aminotransferase assay reagents. |
| Performance Range / Specifications | Pyruvate kinase: ≤0.5%; lactate dehydrogenase: ≤0.01%; glutamate oxaloacetate transaminase: ≤0.01%. |
| Storage Conditions | Store without agitation at -25°C to -15°C. |
| Shelf Life | Retains at least 90% activity for 12 months under the stated storage conditions. |
| Package Specifications | Available in 20 kU, 100 kU and 1,000 kU packs. |
| Product Form | Lyophilized enzyme powder |
| Activity / Unit Definition | One unit is the amount of enzyme that consumes 1 µmol of NADH per minute under the specified reaction conditions. |
| Molecular Weight | 35 kDa by SDS-PAGE |
| Source / Origin | Microbial |
| pH Range / Optimal pH | Optimum pH: 8.0-9.0; stable at pH 4.0-9.0 after 16 hours at 25°C. |
| Appearance | White amorphous lyophilized powder |
| Alias | Malate dehydrogenase |
| Product Type | Diagnostic raw-material enzyme |
| Function / Efficacy | Catalyzes the malate/oxaloacetate redox reaction with NADH participation for coupled biochemical assays. |
| Abbreviation | MDH |
| EC Number | EC 1.1.1.37 |
| Specific Activity | ≥1,300 U/mg enzyme powder |
| Isoelectric Point | 5.61 |
| Inhibitors | Hg2+ |
| Optimal Temperature | 60°C |
| Thermal Stability | Stable below 50°C at pH 7.5 for 30 minutes. |
| Michaelis Constant | 7.8 × 10^-5 M for oxaloacetate; 1.2 × 10^-4 M for NADH. |
For research use only, not for clinical use.
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