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| Product Name | >=130 U/mg Creatinine Amidohydrolase for 520 nm Creatinine Assays |
| Catalog No. | IEC-HMM-0169 |
| Description | Alta DiagnoTech creatinine amidohydrolase is a gray-brown amorphous enzyme powder for creatinine reagent development. |
| Intended Use | Enzyme raw material for creatinine assays based on a sarcosine-oxidase reaction sequence. |
| Detection Method | Endpoint colorimetric assay at 520 nm |
| Performance Range / Specifications | Specific activity >=130 U/mg; stated assay range 1.8-2.4 U/mL. |
| Reaction Conditions / Protocol | Incubate 1.0 mL of 0.1 M creatine solution in 500 mM potassium phosphate at pH 7.5 for 5 minutes at 37 degrees C, add 0.1 mL sample and react for 10 minutes. Transfer 0.1 mL reaction mixture to 2.0 mL of 0.5 M NaOH, add 1.0 mL of 1% picric acid, incubate for 20 minutes at 25 degrees C and read at 520 nm against a dilution-buffer blank. |
| Storage Conditions | Store at -20 degrees C |
| Shelf Life | At least 1 year at -20 degrees C |
| Product Form | Gray-brown amorphous powder |
| Activity / Unit Definition | One unit forms 1 umol of the orange-colored product per minute under the stated reaction conditions. |
| Molecular Weight | Approximately 28 kDa |
| pH Range / Optimal pH | Stable at pH 6.0-10.0; optimum pH 8.0 |
| Appearance | Gray-brown amorphous powder |
| Alias | Creatinine amidohydrolase |
| Product Type | Creatinine amidohydrolase enzyme raw material |
| Function / Efficacy | Provide creatinine-amidohydrolase activity for enzymatic creatinine testing. |
| Abbreviation | CAH |
| Customization | Specifications can be customized |
| Specific Activity | >=130 U/mg |
| Isoelectric Point | 4.70 |
| Inhibitors | Not inhibited by sodium azide |
| Optimal Temperature | 45 degrees C |
| Thermal Stability | Stable below 65 degrees C |
| Michaelis Constant | 5 x 10^-2 M for creatinine |
| Stabilizer | Mg2+ |
| Detection Wavelength | 520 nm |
| Linear Range | 1.8-2.4 U/mL |
For research use only, not for clinical use.
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