>=12 U/mg L-Glycerol-3-Phosphate Oxidase for Triglyceride Reagents

Name: >=12 U/mg L-Glycerol-3-Phosphate Oxidase for Triglyceride Reagents
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Cat.No: IEC-HMM-0227 Datasheet

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Product Name	>=12 U/mg L-Glycerol-3-Phosphate Oxidase for Triglyceride Reagents
Catalog No.	IEC-HMM-0227
Description	Alta DiagnoTech L-glycerol-3-phosphate oxidase is a yellow amorphous lyophilized enzyme powder for coupled enzymatic triglyceride assays.
Intended Use	Used with lipoprotein lipase and glycerol kinase for enzymatic triglyceride reagent development.
Performance Range / Specifications	Specific activity >=12 U/mg dry powder; protein purity >=90%; cholesterol oxidase <=0.001%; lactate oxidase <=0.002%; uricase <=0.05%.
Package Specifications	5 kU, 100 kU, 500 kU or 1,000 kU
Product Form	Yellow amorphous lyophilized powder
Purity	>=90% protein purity
Activity / Unit Definition	One unit is the amount of enzyme that consumes 1 umol glycerol-3-phosphate per minute under the stated conditions.
Molecular Weight	75 kDa by SDS-PAGE
Source / Origin	Microbial source
pH Range / Optimal pH	Optimum pH 8.0-8.5; stable at pH 5.5-9.5 for 16 hours at 25 degrees C
Recommended Buffer System	pH profiling: acetate at pH 4.5-5.5, phosphate at pH 6.0-8.0, Tris-HCl at pH 8.0-9.0 and glycine-NaOH at pH 9.0-10.0. Temperature and thermal-stability studies use 20 mM Tris-HCl at pH 7.5 and 1 mg/mL enzyme.
Appearance	Yellow amorphous lyophilized powder
Alias	L-Glycerol-3-Phosphate Oxidase
Product Type	L-glycerol-3-phosphate oxidase enzyme raw material
Function / Efficacy	Oxidize glycerol-3-phosphate in coupled triglyceride assay systems.
Abbreviation	G3PO
EC Number	EC 1.1.3.21
Specific Activity	>=12 U/mg dry powder
Isoelectric Point	Approximately 4.2
Inhibitors	Fe3+
Optimal Temperature	55 degrees C
Thermal Stability	Stable below 45 degrees C

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For research use only, not for clinical use.