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| Product Name | 10±1 mg/mL Recombinant Alkaline Phosphatase ≥2,000 U/mg for Glycan Coupling |
| Catalog No. | IEC-HMM-0144 |
| Description | Alta DiagnoTech recombinant alkaline phosphatase is a high-activity, heat-stable homodimeric phosphomonoester hydrolase supplied as a clear liquid for immunoassay conjugation. |
| Intended Use | As a labeling enzyme for antibody and antigen conjugates used in colorimetric, fluorescent, luminescent and immunohistochemical assays; coupling through glycosylated side chains is recommended. |
| Principle / Technology | The enzyme catalyzes hydrolysis of phosphate monoesters under alkaline conditions to produce orthophosphate and the corresponding alcohol, phenol or sugar. |
| Storage Conditions | Store sealed at 2°C to 8°C. |
| Product Form | Colorless clear enzyme solution |
| Purity | ≥95% by HPLC |
| Concentration | 10±1 mg/mL protein |
| Molecular Weight | 140 kDa total; 110 kDa protein sequence and 30 kDa glycan portion (27%) |
| Source / Origin | Recombinant Pichia pastoris carrying a calf-intestinal alkaline-phosphatase gene |
| Application Notes / Precautions | For in vitro reagent manufacturing only. Wear protective clothing and gloves during handling. |
| Appearance | Colorless clear liquid |
| Alias | Alkaline Phosphatase |
| Product Type | Recombinant alkaline phosphatase labeling enzyme |
| Function / Efficacy | Provides alkaline-phosphatase activity for sensitive immunoassay labels. |
| Abbreviation | ALP |
| Functional Group | 8 primary amines |
| EC Number | 3.1.3.1 |
| Specific Activity | ≥2,000 U/mg by the glycine method |
| Storage Buffer | 3 mol/L NaCl, 5 mmol/L MgCl2, 0.1 mmol/L ZnCl2 and 30 mmol/L triethanolamine, pH 7.6 |
For research use only, not for clinical use.
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