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| Product Name | >=100 U/mg Cholesterol Esterase for HDL-C and LDL-C Reagents |
| Catalog No. | IEC-HMM-0164 |
| Description | Alta DiagnoTech cholesterol esterase is a white to light-brown amorphous lyophilized enzyme powder for high- and low-density lipoprotein cholesterol assays. |
| Intended Use | Enzyme raw material for research and bulk formulation of high- and low-density lipoprotein cholesterol reagents. |
| Performance Range / Specifications | Specific activity >=100 U/mg dry powder; protein purity >=90.0%; catalase <=0.001%; protease <=0.001%; uricase <=0.01%; ATPase <=0.005%; glycerol kinase <=0.01%; glucose oxidase <=0.01%. |
| Storage Conditions | Store at -25 to -15 degrees C |
| Shelf Life | Maintains at least 90% activity for 12 months at -25 to -15 degrees C |
| Package Specifications | 2 kU, 100 kU, 500 kU or 1,000 kU |
| Product Form | White to light-brown amorphous lyophilized powder |
| Purity | >=90.0% protein purity |
| Activity / Unit Definition | One unit is the amount of enzyme associated with generation of 1 umol hydrogen peroxide per minute under the stated coupled conditions. |
| Molecular Weight | 61 kDa by SDS-PAGE |
| Source / Origin | Microbial source |
| pH Range / Optimal pH | Optimum pH 5.0; stable at pH 4.5-9.0 for 60 minutes at 37 degrees C |
| Appearance | White to light-brown amorphous lyophilized powder |
| Alias | Cholesterol Esterase |
| Product Type | Cholesterol esterase enzyme raw material |
| Function / Efficacy | Hydrolyze cholesterol esters in coupled lipoprotein cholesterol assays. |
| Abbreviation | CE |
| EC Number | EC 3.1.1.13 |
| Specific Activity | >=100 U/mg dry powder |
| Isoelectric Point | 5.15 |
| Inhibitors | Hg2+ and Ag+ |
| Optimal Temperature | 55 degrees C |
| Thermal Stability | Stable below 50 degrees C at pH 7.5 for 30 minutes |
| Michaelis Constant | 5.8 x 10^-5 M for cholesterol linoleate |
| Stabilizer | Sodium cholate and BSA |
For research use only, not for clinical use.
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