>=1.0 U/mg Uricase for 505 nm Uric Acid Colorimetric Assays

Name: >=1.0 U/mg Uricase for 505 nm Uric Acid Colorimetric Assays
Price: Contact Alta DiagnoTech for price USD
Availability: InStock

Cat.No: IEC-HMM-0260 Datasheet

Quantities:

- +

Add To Cart

Product Details Related Products

Product Name	>=1.0 U/mg Uricase for 505 nm Uric Acid Colorimetric Assays
Catalog No.	IEC-HMM-0260
Description	Alta DiagnoTech uricase is a pale-yellowish white amorphous enzyme powder for uric-acid biochemical reagent development.
Intended Use	Enzyme raw material for uric acid biochemical assay reagents.
Detection Method	Peroxidase-coupled kinetic colorimetric assay at 505 nm
Performance Range / Specifications	Specific activity >=1.0 U/mg; assay linear and recommended sample range 400-600 U/L.
Reaction Conditions / Protocol	Prepare Reagent 1 by dissolving 6.06 g Tris in 800 mL water, adjusting to pH 8.50 with HCl, adding 6.0 g phenol, 0.3462 g EDTA disodium salt and 1 g sodium azide, adjusting to pH 8.50 +/- 0.05 at 25 degrees C and bringing to 1 L; store for up to 2 weeks at 2-8 degrees C. Prepare Reagent 2 in 700 mL water with 0.8 g lithium carbonate, 30.1 g disodium hydrogen phosphate dodecahydrate, 0.884 g pre-dissolved oxalic acid and 2.5 g sodium dihydrogen phosphate dihydrate; adjust to pH 7.50, add 0.25 g uric acid, 2.34 g 4-aminoantipyrine, 6.0 kU peroxidase and 1 g sodium azide, then bring to 1 L at pH 7.50 +/- 0.05. Combine 0.500 mL Reagent 1 and 2.500 mL Reagent 2, equilibrate at 37 degrees C, add 0.030 mL enzyme sample after 4 minutes, then after 2 minutes determine the 1-minute absorbance-change rate at 505 nm. Activity in U/L equals Delta A/min x 15,188 x dilution factor.
Storage Conditions	Store at -20 degrees C
Shelf Life	At least 1 year at -20 degrees C
Product Form	Pale-yellowish white amorphous powder
Activity / Unit Definition	One unit is the amount of enzyme that produces 1 umol hydrogen peroxide, corresponding to 0.5 umol quinone dye, per minute under the stated reaction conditions.
Molecular Weight	Approximately 31 kDa
pH Range / Optimal pH	Stable at pH 6.5-10.0; optimum pH 8.5-9.5
Recommended Buffer System	Sample diluent: dissolve 2.3 g potassium dihydrogen phosphate and 22.8 g dipotassium hydrogen phosphate in 800 mL water, adjust to pH 7.60, add 1 g BSA and 1 g sodium azide, adjust to pH 7.60 +/- 0.05 at 25 degrees C and bring to 1 L. Store for up to 1 week at 2-8 degrees C.
Appearance	Pale-yellowish white amorphous powder
Alias	Uricase
Product Type	Uricase enzyme raw material
Function / Efficacy	Oxidize uric acid and generate hydrogen peroxide for coupled colorimetric detection.
Abbreviation	UA
Customization	Specifications can be customized
Specific Activity	>=1.0 U/mg
Isoelectric Point	8.4
Inhibitors	Not inhibited by sodium azide
Optimal Temperature	>60 degrees C
Thermal Stability	Stable below 50 degrees C
Michaelis Constant	1.6 x 10^-6 M
Detection Wavelength	505 nm
Linear Range	400-600 U/L

Cat.No	Product Name	Price
IEC-HMM-0238	≥500,000 U/mL PNGase F for Broad N-Glycan Release from Glycoproteins	Add To Cart
IVDRM-0432	Recombinant DNase I, E. coli Expressed, RNase-Free	Add To Cart
IVDRM-0415	EcoRI Rapid Restriction Endonuclease, High Concentration (100 U/uL)	Add To Cart
IEC-HMM-0208	15 U/µL Glycerol-Free 65°C Reverse Transcriptase for RT-LAMP	Add To Cart
IVDRM-0081	Fast Restriction Enzyme SphI (Recognition Site: GCATG/C)	Add To Cart
IEC-HMM-0147	>=10 U/mg Alpha-Glucosidase for 405 nm PNPG Amylase Assays	Add To Cart
IVDRM-0583	High Affinity Porcine RNase Inhibitor, Ultra-Low Residual (40 U/uL)	Add To Cart
IEC-HMM-0143	>=10 U/mg Adenosine Deaminase for 265 nm Adenosine Hydrolysis Assays	Add To Cart
IEC-HMM-0110	High-Sensitivity Warm-Start Bst III DNA Polymerase, Glycerol-Free	Add To Cart
IVDRM-0488	Thermostable RNase H (5 U/uL)	Add To Cart

For research use only, not for clinical use.