>=10 U/mg Sarcosine Oxidase for 500 nm Creatinine Assays

Name: >=10 U/mg Sarcosine Oxidase for 500 nm Creatinine Assays
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Cat.No: IEC-HMM-0255 Datasheet

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Product Name	>=10 U/mg Sarcosine Oxidase for 500 nm Creatinine Assays
Catalog No.	IEC-HMM-0255
Description	Alta DiagnoTech sarcosine oxidase is a yellow amorphous enzyme powder for creatinine reagent development using a hydrogen-peroxide-coupled color reaction.
Intended Use	Enzyme raw material for creatinine assays based on the sarcosine oxidase method.
Detection Method	Kinetic quinone-dye absorbance assay at 500 nm
Performance Range / Specifications	Specific activity >=10 U/mg; assay linear range 100-1,000 U/L.
Reaction Conditions / Protocol	Use a Tris-HCl reaction reagent at pH 8.0 containing 4-aminoantipyrine, phenol and peroxidase, with 1 M sarcosine as substrate. Preincubate 1.0 mL reagent plus 0.012 mL enzyme for 60 seconds at 37 degrees C, add 0.2 mL substrate and determine the 2-minute rate at 500 nm.
Storage Conditions	Store at -20 degrees C
Shelf Life	At least 1 year at -20 degrees C
Product Form	Yellow amorphous powder
Activity / Unit Definition	One unit produces 1 umol hydrogen peroxide, equivalent to 0.5 umol quinone dye, per minute under the stated conditions.
Molecular Weight	43 kDa
pH Range / Optimal pH	Stable at pH 6.5-10.5 for 24 hours at 20 degrees C; optimum pH 7.0-8.0
Recommended Buffer System	20 mM Tris-HCl at pH 8.0 containing 2.0 mM EDTA for sample dilution
Appearance	Yellow amorphous powder
Alias	Sarcosine oxidase
Product Type	Sarcosine oxidase enzyme raw material
Function / Efficacy	Oxidize sarcosine and generate hydrogen peroxide for coupled colorimetric detection.
Abbreviation	SOX
Customization	Specifications can be customized
Specific Activity	>=10 U/mg
Isoelectric Point	5.32
Inhibitors	Not inhibited by sodium azide
Optimal Temperature	50-55 degrees C
Thermal Stability	No activity loss below 60 degrees C for 10 minutes
Michaelis Constant	2.09 x 10^-3 mol/L for sarcosine
Stabilizer	Potassium ions support enzyme stability
Detection Wavelength	500 nm
Linear Range	100-1,000 U/L

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For research use only, not for clinical use.