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| Product Name | >=10 U/mg Cholesterol Oxidase for TCHO, HDL-C and LDL-C Assays |
| Catalog No. | IEC-HMM-0165 |
| Description | Alta DiagnoTech cholesterol oxidase is a pale-yellow amorphous enzyme powder for total cholesterol, HDL-C and LDL-C biochemical reagent development. |
| Intended Use | Enzyme raw material for total cholesterol, HDL cholesterol and LDL cholesterol biochemical assay kits. |
| Detection Method | Peroxidase-coupled quinone-dye absorbance assay at 505 nm |
| Performance Range / Specifications | Specific activity >=10 U/mg; assay linear range 75-1,000 U/L with 200-400 U/L recommended. |
| Reaction Conditions / Protocol | Use potassium phosphate reagents at pH 7.0 containing phenol, cholesterol, sodium cholate, BSA, 4-aminoantipyrine and peroxidase. Mix 0.6 mL reagent 1 and 0.6 mL reagent 2, preincubate for 60 seconds at 37 degrees C, add 0.012 mL sample after 4 minutes and measure the one-minute rate at 505 nm. Reprepare reagent 2 if cholesterol precipitation causes turbidity. |
| Storage Conditions | Store at -20 degrees C |
| Shelf Life | At least 1 year at -20 degrees C |
| Product Form | Pale-yellow amorphous powder |
| Activity / Unit Definition | One unit produces 1 umol hydrogen peroxide, equivalent to 0.5 umol quinone dye, per minute under the stated conditions. |
| Molecular Weight | Approximately 62 kDa |
| pH Range / Optimal pH | Stable at pH 6.0-10.0; optimum pH 6.5-7.5 |
| Appearance | Pale-yellow amorphous powder |
| Alias | Cholesterol oxidase |
| Product Type | Cholesterol oxidase enzyme raw material |
| Function / Efficacy | Oxidize cholesterol and generate hydrogen peroxide for coupled colorimetric detection. |
| Abbreviation | COD |
| Customization | Specifications can be customized |
| Specific Activity | >=10 U/mg |
| Isoelectric Point | 5.46 |
| Inhibitors | Not inhibited by sodium azide |
| Optimal Temperature | 52 degrees C |
| Thermal Stability | Stable below 60 degrees C |
| Michaelis Constant | 1 x 10^-4 M for cholesterol |
| Stabilizer | Potassium ions support enzyme stability |
| Detection Wavelength | 505 nm |
| Linear Range | 75-1,000 U/L; 200-400 U/L recommended |
For research use only, not for clinical use.
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