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| Product Name | >=10 U/mg Acetyl-CoA Oxidase for 500 nm Free Fatty Acid Assays |
| Catalog No. | IEC-HMM-0139 |
| Description | Alta DiagnoTech acetyl-CoA oxidase is a pale-yellow amorphous enzyme powder for free-fatty-acid biochemical reagent development. |
| Intended Use | Enzyme raw material for non-esterified fatty acid biochemical assay kits. |
| Detection Method | Peroxidase-coupled quinone-dye absorbance assay at 500 nm |
| Performance Range / Specifications | Specific activity >=10 U/mg; assay linear range 0.2-0.5 kU/L. |
| Reaction Conditions / Protocol | Use Tris buffer at pH 8.0 with 4-aminoantipyrine, phenol, peroxidase, Triton X-100 and palmitoyl-CoA. Combine 0.2 mL buffer, 0.1 mL each of 4-AA, phenol, peroxidase, Triton X-100 and substrate plus 0.3 mL water; preincubate for 300 seconds at 37 degrees C, add 0.02 mL sample and determine the one-minute rate at 500 nm. The enzyme dilution buffer contains FAD and ATP. |
| Storage Conditions | Store at -20 degrees C |
| Shelf Life | At least 1 year at -20 degrees C |
| Product Form | Pale-yellow amorphous powder |
| Activity / Unit Definition | One unit produces 1 umol hydrogen peroxide, corresponding to 0.5 umol quinone dye, per minute under the stated conditions. |
| Molecular Weight | Approximately 75 kDa |
| pH Range / Optimal pH | Stable at pH 6.0-8.5 for 2 hours at 37 degrees C; optimum pH 7.0-9.0 |
| Appearance | Pale-yellow amorphous powder |
| Alias | Acetyl-CoA oxidase |
| Product Type | Acetyl-CoA oxidase enzyme raw material |
| Function / Efficacy | Generate hydrogen peroxide from acyl-CoA substrate for free-fatty-acid detection. |
| Abbreviation | ACOD |
| Customization | Specifications can be customized |
| Specific Activity | >=10 U/mg |
| Isoelectric Point | 6.0 |
| Inhibitors | Not inhibited by sodium azide |
| Optimal Temperature | 35-50 degrees C |
| Thermal Stability | Stable below 45 degrees C for 15 minutes |
| Detection Wavelength | 500 nm |
| Linear Range | 0.2-0.5 kU/L |
For research use only, not for clinical use.
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