>=1 kU/mg Neuraminidase for Coupled 340 nm Sialic Acid Assays

Name: >=1 kU/mg Neuraminidase for Coupled 340 nm Sialic Acid Assays
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Cat.No: IEC-HMM-0237 Datasheet

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Product Name	>=1 kU/mg Neuraminidase for Coupled 340 nm Sialic Acid Assays
Catalog No.	IEC-HMM-0237
Description	Alta DiagnoTech neuraminidase, also known as sialidase, is a white amorphous enzyme powder for sialic acid reagent development.
Intended Use	Enzyme raw material for biochemical sialic acid assay kits.
Detection Method	NAL/LDH-coupled NADH absorbance assay at 340 nm
Performance Range / Specifications	Specific activity >=1 kU/mg; assay linear range 200-500 U/L.
Reaction Conditions / Protocol	Use 50 mM potassium phosphate at pH 7.5 with 8.0 mM sialyllactose, 100 U/mL N-acetylneuraminic acid aldolase, 100 U/mL L-lactate dehydrogenase and 2.5 mM NADH. Combine 125 uL buffer, 300 uL substrate, 50 uL NADH, 62.5 uL NAL and 62.5 uL LDH, incubate for 2 minutes at 37 degrees C, add 25 uL sample and calculate the rate from minutes 7-10 at 340 nm. Keep the sialyllactose reagent cold and uncontaminated.
Storage Conditions	Store at -20 degrees C
Shelf Life	At least 1 year at -20 degrees C
Product Form	White amorphous powder
Activity / Unit Definition	One unit hydrolyzes 1 umol sialic acid per minute under the stated assay conditions.
Molecular Weight	42 kDa
pH Range / Optimal pH	Stable at pH 4.0-9.5; optimum pH 7.0
Appearance	White amorphous powder
Alias	Sialidase
Other Name	Neuraminidase
Product Type	Neuraminidase enzyme raw material
Function / Efficacy	Release sialic acid from sialylated substrate for coupled measurement.
Abbreviation	SA/NA
Customization	Specifications can be customized
Specific Activity	>=1 kU/mg
Isoelectric Point	8.5
Optimal Temperature	55 degrees C
Thermal Stability	Stable below 42 degrees C
Michaelis Constant	2.1 x 10^-3 M for sialyllactose
Detection Wavelength	340 nm
Linear Range	200-500 U/L

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For research use only, not for clinical use.