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| Product Name | 1-10 kU/L Recombinant Acid Phosphatase for ACP Calibration and QC |
| Catalog No. | IEC-HMM-0142 |
| Description | Alta DiagnoTech recombinant acid phosphatase is supplied as a nearly colorless clear solution for ACP assay calibration and quality control. |
| Intended Use | Calibrator or quality-control material for acid phosphatase assay kits. |
| Principle / Technology | ACP hydrolyzes alpha-naphthyl phosphate to alpha-naphthol, which then forms a diazo compound with 2-amino-5-chlorotoluene. |
| Detection Method | Acid phosphatase enzyme activity assay |
| Performance Range / Specifications | Activity is 1-10 kU/L, with the exact value stated on the test report and container label or set to customer requirements. Specific activity is approximately 13 U/mg. |
| Storage Conditions | Stable for at least 6 months at 2-8 degrees C; store at -20 degrees C for long-term preservation and avoid repeated freeze-thaw cycles |
| Shelf Life | At least 6 months at 2-8 degrees C |
| Product Form | Nearly colorless clear solution |
| Purity | SDS-PAGE shows an approximately 25 kDa band without obvious additional bands |
| Activity / Unit Definition | At 37 degrees C, one unit is the amount of enzyme that generates 1 umol alpha-naphthol per minute. |
| Molecular Weight | Approximately 25 kDa by SDS-PAGE |
| Source / Origin | Recombinant expression in Escherichia coli |
| pH Range / Optimal pH | Stable at pH 6.5-12.0 |
| Appearance | Nearly colorless clear solution |
| Alias | Acid Phosphatase |
| Safety | Contains sodium azide preservative; use appropriate precautions. No other known biological hazards are stated. |
| Product Type | Acid phosphatase calibrator and QC raw material |
| Function / Efficacy | Hydrolyze alpha-naphthyl phosphate for ACP assay calibration and control. |
| Abbreviation | ACP |
| Customization | Activity and specifications can be customized |
| EC Number | EC 3.1.3.2 |
| Specific Activity | Approximately 13 U/mg |
| Inhibitors | EDTA |
| Thermal Stability | Retains more than 90% activity after 30 minutes at 42 degrees C; activity decreases rapidly above 50 degrees C |
| Activator | Mg2+; optimal Mg2+ concentration is 200 mM in diluent and 25 mM in the activity reagent |
| Expression Host | Escherichia coli |
| Chemical Stability | Tolerates 200 mM L-tartrate |
For research use only, not for clinical use.
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