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| Product Name | V Multiplex One-Step RT-qPCR Probe Kit |
| Catalog No. | RESK-0095 |
| Description | This kit is designed for multiplex quantitative PCR using RNA as the template. Reverse transcription and quantitative PCR are performed in the same reaction tube, simplifying handling and reducing the risk of contamination. A thermostable reverse transcriptase efficiently synthesizes first-strand cDNA, and a hot-start Taq DNA polymerase drives quantitative amplification. The kit contains an optimized MP Buffer and an Enzymes Mix; the buffer already includes Mg2+ and dNTPs, together with factors that effectively suppress non-specific PCR amplification and enhance multiplex qPCR efficiency, enabling up to 4-plex reactions in a single tube while maintaining primer amplification efficiency. The kit is well suited for multiplex detection of RNA viruses and other RNA pathogens. |
| Intended Use | Multiplex probe-based RT-qPCR detection and quantification of RNA targets, including RNA viruses and other RNA pathogens, in research applications. |
| Principle / Technology | One-step probe-based RT-qPCR: a thermostable reverse transcriptase first converts RNA into first-strand cDNA, followed by real-time quantitative amplification by a hot-start Taq DNA polymerase in the same tube. Target-specific hydrolysis probes generate fluorescent signals during amplification. The optimized buffer contains additives that suppress non-specific amplification and improve multiplex qPCR efficiency, supporting up to 4-plex detection. |
| Detection Method | Real-time quantitative PCR with fluorescent hydrolysis probes (probe-based multiplex RT-qPCR) |
| Sample Type | RNA templates, including RNA extracted from viral or other pathogen samples |
| Performance Range / Specifications | Reverse transcription and quantitative PCR performed in one tube for simple operation; supports multiplex detection of up to 4 targets in a single reaction. |
| Specificity | The buffer contains factors that effectively inhibit non-specific PCR amplification, ensuring reliable target specificity in multiplex reactions. |
| Reaction Conditions / Protocol | One-tube protocol: reverse transcription and quantitative PCR are performed sequentially in the same reaction tube without intermediate handling. |
| Components / Formulation | Optimized MP Buffer (contains Mg2+ and dNTPs, plus factors that suppress non-specific amplification and enhance multiplex qPCR efficiency) Enzymes Mix (thermostable reverse transcriptase and hot-start Taq DNA polymerase) |
| Storage Conditions | Store at -25 to -15°C. |
| Shelf Life | 1 year when stored at -25 to -15°C. |
| Package Specifications | 100 T 1000 T 10000 T 50 T |
| Product Form | Liquid kit (buffer and enzyme mix) |
| Expiration Date / Stability | Stable for 1 year at -25 to -15°C. |
| Regulatory / Compliance | For research use only. |
| Compatibility | Supports up to 4-plex probe-based detection in a single reaction; compatible with mainstream real-time PCR instruments. |
For research use only, not for clinical use.
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