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| Product Name | C64P1 TaqMan Multiplex qPCR Kit (Split Version) |
| Catalog No. | RESK-0099 |
| Description | The C64P1 TaqMan Multiplex qPCR Kit (Split Version) is a real-time quantitative PCR reagent based on a next-generation antibody-based hot-start Taq DNA polymerase. In this split format, the 5× buffer, Mg2+, dNTPs and hot-start Taq polymerase are supplied as separate components, greatly increasing the flexibility of assay development and reaction optimization. The buffer contains factors that effectively suppress non-specific PCR amplification and factors that enhance multiplex amplification efficiency, enabling up to 4-plex reactions while maintaining primer efficiency. |
| Intended Use | Development and optimization of multiplex real-time qPCR assays where independent adjustment of buffer, Mg2+, dNTP and enzyme concentrations is required. |
| Principle / Technology | Probe-based (TaqMan) real-time quantitative PCR using a next-generation antibody-modified hot-start Taq DNA polymerase. Polymerase activity is blocked at ambient temperature and activated by heat, minimizing non-specific amplification and primer-dimer formation. |
| Detection Method | TaqMan probe-based real-time fluorescence quantitative PCR (qPCR). |
| Sample Type | DNA templates. |
| Performance Range / Specifications | Supports up to 4-plex qPCR reactions while maintaining primer amplification efficiency. |
| Specificity | The buffer contains factors that effectively suppress non-specific PCR amplification and factors that enhance amplification efficiency in multiplex qPCR, ensuring primer efficiency in up to 4-plex reactions. |
| Reaction Conditions / Protocol | Split four-component format: combine the 5× buffer, Mg2+ solution, dNTP mix and hot-start Taq polymerase with primers, probes and template; each component can be independently titrated during assay optimization. |
| Components / Formulation | 5× buffer solution Hot-start Taq DNA polymerase solution Mg2+ solution dNTP mix |
| Storage Conditions | Store at -25 to -15°C. |
| Shelf Life | 2 years when stored at -25 to -15°C. |
| Package Specifications | 100 T 1000 T 10000 T |
| Product Form | Split version: four separately packaged liquid components (buffer solution, enzyme solution, Mg2+ solution and dNTP mix) for convenient reaction system optimization. |
| Expiration Date / Stability | Valid for 2 years when stored at -25 to -15°C. |
| Regulatory / Compliance | For research use and in vitro diagnostic reagent development. Not for direct clinical diagnosis. |
| Compatibility | Suitable for mainstream real-time PCR instruments. |
| Recommended Buffer System | Supplied 5× buffer with separately adjustable Mg2+ and dNTP concentrations. |
For research use only, not for clinical use.
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