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| Product Name | Advanced Fast 2-Step RT-qPCR Probe Kit |
| Catalog No. | RESK-0077 |
| Description | The Advanced Fast 2-Step RT-qPCR Probe Kit is designed for reverse transcription and amplification of RNA templates rapidly released from cultured cell lysates. It comprises a reverse transcription reagent (RT SuperMix) and a probe-based qPCR reagent (2x TaqMan qPCR mix), offering strong system tolerance and broad compatibility. The kit supports high-throughput (96/384-well) cellular RNA expression analysis. The 100 T format provides 96 x 2 qPCR reactions, sufficient for one complete 96-well cell plate in a single use, avoiding contamination and performance loss from repeated opening and freeze-thaw cycles. The 500 T format is suited to 384-well and higher-throughput automated screening platforms. |
| Intended Use | Two-step, probe-based reverse transcription and quantitative PCR for high-throughput gene expression analysis from cell lysates. For research use only. |
| Principle / Technology | Two-step RT-qPCR with probe-based (TaqMan) detection: RNA in cell lysates is first reverse-transcribed into cDNA with RT SuperMix, then amplified and quantified with a 2x probe-based qPCR master mix. Separating the two reactions facilitates cDNA storage and traceability. |
| Detection Method | Probe-based (TaqMan) quantitative real-time PCR fluorescence detection. |
| Sample Type | RNA released from cultured cell lysates (compatible with adherent cells such as 293T and suspension cells such as 293F). |
| Performance Range / Specifications | Separate reverse transcription and amplification steps facilitate cDNA storage and traceability. High reagent tolerance: withstands up to 30% cell lysate in the reaction. High throughput: up to 384-well gene expression screening in a single run, more than 4x the throughput of conventional methods. High precision: contains universal ROX reference dye and is compatible with a wide range of qPCR instruments. |
| Sensitivity / LOD | Delivers stable and highly sensitive detection from cell lysate templates, reducing the risk of false negatives (missed detection) compared with conventional products. |
| Reaction Conditions / Protocol | Step 1: Reverse transcription of RNA in cell lysate using RT SuperMix. Step 2: Probe-based qPCR amplification of the resulting cDNA using 2x TaqMan qPCR mix. The 100 T format provides 96 x 2 qPCR reactions, sufficient for one full 96-well plate. |
| Components / Formulation | RT SuperMix (reverse transcription reagent) 2x TaqMan qPCR mix (probe-based qPCR reagent, containing universal ROX reference dye) |
| Storage Conditions | Store at -25°C to -15°C. |
| Shelf Life | 1 year when stored as recommended. |
| Package Specifications | 100 T; 500 T |
| Quality Control | Validated on multiple target genes in 293T adherent and 293F suspension cells, demonstrating effective amplification, stable and sensitive detection with reduced missed-detection rates, and high whole-plate precision with minimal systematic error and good uniformity. |
| Expiration Date / Stability | Valid for 1 year at -25°C to -15°C. |
| Compatibility | Contains universal ROX reference dye, making it compatible with a wide range of qPCR instruments (both ROX-dependent and ROX-independent models). Broad sample compatibility, validated on 293T adherent cells and 293F suspension cells. Suitable for 96/384-well high-throughput automated screening platforms. |
| Application Notes / Precautions | The reaction system tolerates up to 30% cell lysate. Use the 100 T format for one complete 96-well plate in a single session to avoid contamination and reagent performance degradation caused by repeated opening and freeze-thaw cycles. |
| Batch-to-Batch Consistency | Whole-plate detection achieved in a single run shows small systematic error and good uniformity. |
For research use only, not for clinical use.
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