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| Product Name | RT-LAMP Lyo-Ready Fluorescent Assay Kit (Bst Plus) |
| Catalog No. | RESK-0121 |
| Description | The RT-LAMP Lyo-Ready Fluorescent Assay Kit (Bst Plus) is a second-generation fluorescent dye-based RT-LAMP kit capable of isothermal amplification from both DNA and RNA templates. It combines a new-generation aptamer-blocked Bst Plus DNA Polymerase, a heat-stable reverse transcriptase, and murine RNase inhibitor within a finely optimized buffer system, enabling highly efficient, rapid, and specific detection reactions, and supports reaction setup at room temperature. Both the buffer and enzyme components are glycerol-free and, together with the lyoprotectant provided, are suitable for lyophilization. |
| Intended Use | Rapid isothermal amplification and fluorescent detection of DNA and RNA targets; designed for development of lyophilized molecular diagnostic reagents and pathogen testing applications. For research use. |
| Principle / Technology | Loop-mediated isothermal amplification (LAMP/RT-LAMP) driven by an aptamer-blocked Bst Plus DNA Polymerase and a heat-stable reverse transcriptase, allowing DNA and RNA templates to be amplified at a constant temperature. Glycerol-free formulation supports lyophilized product preparation. |
| Detection Method | Fluorescent dye-based detection. An intercalating fluorescent dye (e.g., SYBR Green or Syto-type dyes) binds double-stranded DNA during amplification, increasing the fluorescence signal 800-1,000-fold; amplification is monitored in real time on a fluorescence quantitative PCR instrument. |
| Sample Type | Extracted DNA or RNA from pathogen samples (e.g., African swine fever virus nucleic acid, influenza B virus controls). |
| Performance Range / Specifications | Versatility: validated with more than 20 animal- and human-related pathogen targets with GC contents of 30%-65%, delivering rapid and consistent time-to-peak. Stability: performance is unaffected after 20 freeze-thaw cycles or 7 days of accelerated storage at 37 °C; supports reaction setup at room temperature. Lyophilization-ready: enzyme components are glycerol-free; a lyoprotectant and lyophilization procedure are provided for preparation of lyophilized products. |
| Sensitivity / LOD | African swine fever virus (ASFV) nucleic acid extracted from real samples: down to 15 copies/reaction. Influenza B virus quality control material: down to 10 copies/reaction. Positive detection is defined as a signal peak arising within 30 min. |
| Specificity | Validated across multiple DNA and RNA targets; no non-specific amplification in no-template controls (NTC) within 60 min. |
| Reaction Conditions / Protocol | Isothermal incubation at 60-65 °C on a real-time fluorescence PCR instrument; results are typically available within approximately 30 min. Reaction setup can be performed at room temperature. A lyophilization procedure is provided for preparation of freeze-dried reagents. |
| Components / Formulation | Aptamer-blocked Bst Plus DNA Polymerase (glycerol-free) Heat-stable reverse transcriptase Murine RNase inhibitor Optimized glycerol-free reaction buffer system Fluorescent dye component (Component D) Lyoprotectant |
| Storage Conditions | Store at -25 °C to -15 °C. Component D must be protected from light. |
| Shelf Life | 1 year |
| Package Specifications | 100 T; 1000 T |
| Product Form | Glycerol-free liquid reagent kit, lyophilization-ready (lyoprotectant included) |
| Expiration Date / Stability | Stable for 1 year when stored at -25 °C to -15 °C. Performance is unaffected after 20 freeze-thaw cycles or 7 days at 37 °C (accelerated stability testing), with positive detection maintained and no non-specific amplification. |
| Compatibility | Compatible with real-time fluorescence (quantitative) PCR instruments. |
| Application Notes / Precautions | Protect Component D from light. Reaction setup can be performed at room temperature. Buffer and enzyme components are glycerol-free and can be lyophilized using the supplied lyoprotectant and lyophilization procedure. |
For research use only, not for clinical use.
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