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| Product Name | Universal TaqMan Pro U+ qPCR Mix (One Tube) |
| Catalog No. | RESK-0115 |
| Description | A multiplex fluorescent quantitative PCR (qPCR) master mix built on a next-generation antibody-based hot-start Taq DNA polymerase. This universal, fully pre-mixed one-tube reagent delivers excellent amplification performance with the majority of primer-probe sets, supports primers and probes pre-mixed in advance, and is compatible with fast-cycling programs (detection time 35 min or less). The 2x mix contains factors that suppress non-specific PCR amplification and enhance multiplex amplification efficiency, improving both sensitivity and specificity. A dUTP/UDG carryover contamination control system, in which dUTP replaces dTTP, effectively prevents aerosol contamination and ensures the accuracy of detection results. |
| Intended Use | For DNA-template multiplex real-time quantitative PCR (qPCR) amplification in research and molecular diagnostic assay development; validated across pathogen, human, and plant targets. |
| Principle / Technology | TaqMan probe-based real-time quantitative PCR using a next-generation antibody-based hot-start Taq DNA polymerase. The dUTP/UDG anti-contamination system substitutes dUTP for dTTP so that uracil-DNA glycosylase (UDG) degrades carryover amplicons before amplification, eliminating aerosol contamination risk. |
| Detection Method | Real-time fluorescence detection with TaqMan hydrolysis probes; supports multiplex (3-plex and 4-plex validated) amplification. |
| Sample Type | DNA templates; validated on pathogen, human-derived, and plant targets. |
| Performance Range / Specifications | Universal compatibility: excellent amplification performance with the majority of primer-probe sets. Full premix stability: amplification performance remains unchanged after 14 days of accelerated storage at 37 °C with primers and probes pre-mixed. Fast-cycling support: detection time 35 min or less (results in 30 min under a fast program). 2x mix formulation contains factors that suppress non-specific PCR amplification and enhance multiplex qPCR efficiency, increasing multiplex sensitivity and specificity. dUTP/UDG anti-contamination system prevents aerosol contamination and ensures result accuracy. |
| Sensitivity / LOD | Amplification efficiency above 99% across 7 gradient samples from 1 to 10^6 copies/uL under both standard and fast programs. |
| Specificity | The 2x mix contains factors that effectively suppress non-specific PCR amplification, improving the specificity of multiplex amplification. |
| Reaction Conditions / Protocol | Supports both standard (approx. 1 h 6 min) and fast (30 min) cycling programs. Fast program maintains amplification efficiency above 99%, with Ct deviation (delta Ct) within +/-0.5 and fluorescence deviation within +/-10% versus the standard program; amplification curves show a typical sigmoidal (S) shape. Validated on standard real-time PCR instruments. |
| Components / Formulation | 2x one-tube premix containing antibody-based hot-start Taq DNA polymerase, dNTPs (dUTP in place of dTTP), UDG, reaction buffer, and additives that suppress non-specific amplification and enhance multiplex amplification efficiency. |
| Storage Conditions | Store at -25 to -15 °C. |
| Shelf Life | 18 months. |
| Package Specifications | 100 T; 1,000 T; 10,000 T; 15,000 T; 20,000 T |
| Product Form | 2x liquid premix (one-tube master mix). |
| Expiration Date / Stability | Valid for 18 months when stored at -25 to -15 °C. Full premix shows no change in amplification performance after 7 days at 4 °C or 37 °C, after 50 freeze-thaw cycles, or after 14 days of accelerated storage at 37 °C; Ct deviation (delta Ct) versus the -20 °C control remains within +/-0.5 and fluorescence deviation within 15% across 3-plex and 4-plex amplification systems. |
| Compatibility | Compatible with the majority of primer-probe sets and with standard and fast-cycling programs; validated on standard real-time PCR instruments. |
| Application Notes / Precautions | Primers and probes can be pre-mixed with the reagent in advance without loss of performance. Use UDG pretreatment to eliminate carryover contamination from previous dUTP-containing amplicons. |
For research use only, not for clinical use.
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