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| Product Name | Universal TaqMan Multiplex qPCR Master Mix (UDG Plus) |
| Catalog No. | RESK-0085 |
| Description | This product is a 2x premixed reagent that enables up to four-plex fluorescence quantitative PCR in a single reaction well. It contains a genetically engineered, antibody-based hot-start Taq DNA polymerase that greatly improves amplification sensitivity and specificity, and its multiplex reaction buffer system has been deeply optimized to increase amplification efficiency and promote efficient amplification of low-concentration templates. The incorporated uracil-DNA glycosylase (UDG) effectively eliminates the risk of aerosol-borne carryover contamination. It is suitable for genotyping and multiplex gene quantification analysis. |
| Intended Use | For probe-based multiplex real-time quantitative PCR applications, including genotyping and multiplex gene quantification, where carryover contamination control is required. Suitable as a raw material for molecular diagnostic assay development and research use. |
| Principle / Technology | TaqMan probe-based real-time quantitative PCR using a genetically engineered antibody-mediated hot-start Taq DNA polymerase, combined with a UDG-based anti-contamination system that degrades uracil-containing carryover amplicons before amplification. |
| Detection Method | Fluorescence-based real-time quantitative PCR (TaqMan hydrolysis probe detection). |
| Sample Type | DNA templates, including inhibitor-containing crude samples. Tolerates whole blood at up to 5% of the reaction volume. |
| Performance Range / Specifications | Up to 4-plex amplification in a single reaction well Strong tolerance to blood impurities - compatible with samples containing up to 5% blood Anti-contamination system - UDG effectively prevents aerosol-borne carryover contamination Excellent storage stability - consistent performance over 24 months Broad platform compatibility - validated on mainstream real-time PCR instruments |
| Specificity | The genetically engineered antibody-based hot-start Taq DNA polymerase suppresses non-specific amplification, while the UDG system eliminates carryover amplicons to reduce false positives. |
| Reaction Conditions / Protocol | A UDG digestion step is performed before thermal cycling to degrade uracil-containing carryover products; followed by hot-start activation and standard qPCR cycling. |
| Components / Formulation | 2x master mix containing a genetically engineered antibody-based hot-start Taq DNA polymerase and uracil-DNA glycosylase (UDG) in an optimized multiplex reaction buffer. |
| Storage Conditions | Store at -25 to -15°C. |
| Shelf Life | 2 years when stored at -25 to -15°C. |
| Package Specifications | 1 mL 5 x 1 mL 20 x 5 mL 125 mL 1 x 12.5 mL 10 mL |
| Product Form | Liquid, 2x concentrated premixed master mix. |
| Quality Control | UDG digestion efficiency is verified by amplification of uracil-containing templates (e.g., a uracil-containing plasmid at 1.4 copies/uL), confirming complete digestion of carryover templates compared with a non-UDG control formulation. |
| Expiration Date / Stability | Stable for 24 months at -25 to -15°C with consistent performance. |
| Compatibility | Compatible with mainstream real-time PCR instruments, across mainstream platforms. Suitable for multiplex probe-based qPCR assays. |
| Recommended Buffer System | The 2x master mix contains a fully optimized multiplex reaction buffer; no additional buffer is required. Use with dUTP-containing nucleotide formulations to enable UDG carryover control. |
| Application Notes / Precautions | The UDG anti-contamination system requires uracil-containing amplicons to be effective; it does not protect against contamination by natural (thymine-containing) template DNA. Avoid prolonged exposure of the reaction mixture to elevated temperatures before the UDG digestion step is completed. |
For research use only, not for clinical use.
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