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| Product Name | Universal TaqMan Multiplex qPCR Master Mix (UDG Plus, 5x Concentrate) |
| Catalog No. | RESK-0108 |
| Description | A highly sensitive 5x universal TaqMan multiplex qPCR master mix built on a genetically engineered antibody-based hot-start Taq DNA polymerase. The premix contains the reaction buffer and enzyme blend, uses dUTP in place of dTTP, and incorporates a dUTP/UDG carryover contamination control system that effectively prevents aerosol contamination. Dedicated factors suppress nonspecific PCR amplification and enhance multiplex amplification efficiency, enabling up to 4-plex reactions while maintaining primer efficiency. The 5x concentrated format provides greater flexibility in reaction setup and supports both standard and fast cycling programs. |
| Intended Use | Universal high-sensitivity master mix for probe-based (TaqMan) quantitative real-time PCR, including multiplex assays of up to four targets and rapid cycling protocols. For research use only. |
| Principle / Technology | Probe-based quantitative PCR using a genetically engineered, antibody-blocked hot-start Taq DNA polymerase activated during initial denaturation. A dUTP/UDG carryover prevention system eliminates uracil-containing amplicons from previous reactions. |
| Detection Method | Real-time fluorescence quantitative PCR with hydrolysis (TaqMan) probes |
| Performance Range / Specifications | Outstanding amplification sensitivity: 1 copy/uL stably detected Supports fast cycling programs, with results in as little as 18 min Broad inhibitor tolerance: compatible with templates of 20%-70% GC content and supports simultaneous multi-target detection dUTP/UDG carryover contamination control system Compatible with both standard and fast programs across multiple platforms |
| Sensitivity / LOD | 1 copy/uL stably detected. In performance testing, 5 copies/reaction template was amplified with 20/20 positive detection (100% detection rate). |
| Specificity | Antibody-mediated hot-start mechanism plus nonspecific amplification inhibitors ensure high specificity in multiplex reactions. |
| Reaction Conditions / Protocol | Compatible with standard and rapid qPCR cycling programs; fastest protocol delivers results in approximately 18 min. |
| Components / Formulation | 5x premix containing reaction buffer, genetically engineered antibody hot-start Taq DNA polymerase, UDG, dNTPs (dUTP replacing dTTP), Mg2+, and multiplex amplification enhancers. |
| Storage Conditions | Store at -25°C to -15°C. |
| Shelf Life | 1 year when stored at -25°C to -15°C. |
| Package Specifications | 100 T; 1,000 T; 5,000 T; 10,000 T; 20,000 T |
| Product Form | Liquid 5x master mix |
| Concentration | 5x |
| Expiration Date / Stability | Stable for 1 year at -25°C to -15°C. |
| Regulatory / Compliance | For research use only. Not for use in diagnostic procedures. |
| Compatibility | Compatible with mainstream real-time PCR platforms, including standard and rapid qPCR systems. |
| Application Notes / Precautions | Suitable for templates with 20%-70% GC content and for simultaneous multi-target detection. Use the dUTP/UDG system to control carryover contamination; avoid repeated freeze-thaw cycles. |
For research use only, not for clinical use.
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