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| Product Name | Universal Superfast qPCR Mix (One Tube) |
| Catalog No. | RESK-0128 |
| Description | This product is a fluorescent quantitative qPCR premix built on a new-generation antibody-based hot-start Taq DNA polymerase. It offers broad versatility and supports both fast cycling programs and a full-premix format. In DNA multiplex amplification applications, it delivers the following advantages: (1) excellent universality, with validated amplification performance across a wide range of bacterial, fungal, and viral primers/probes; (2) support for advance pre-mixing of primers and probes, with full-premix amplification performance unchanged after 7 days of accelerated storage at 37 °C; (3) support for fast programs, completing detection in 20 minutes or less; and (4) a dUTP/UDG anti-contamination system in which dUTP replaces dTTP, effectively preventing aerosol contamination and ensuring accurate results. |
| Intended Use | Multiplex quantitative PCR detection of DNA targets (bacteria, fungi, viruses) in high-throughput and rapid testing workflows, including full-premix assay formats. |
| Principle / Technology | Probe/dye-based real-time quantitative PCR using a new-generation antibody-mediated hot-start Taq DNA polymerase; incorporates a dUTP/UDG carry-over prevention system (dUTP in place of dTTP) to eliminate aerosol contamination from previous amplifications. |
| Detection Method | Real-time fluorescent quantitative PCR (qPCR) |
| Sample Type | DNA templates (e.g., bacterial plasmids, viral nucleic acids extracted from real samples such as African swine fever virus) |
| Performance Range / Specifications | 1. Supports fast programs - results in 20 min; 2. Superior full-premix stability - stable after 7 days at 37 °C with pre-mixed primers/probes and after 20 freeze-thaw cycles; 3. Low background residue - no residual E. coli, P. aeruginosa, or human genomic DNA detected across 48 wells; 4. Excellent universality - compatible with diverse multiplex primers/probes (more than 40 target types validated), all stably amplified; 5. High sensitivity and specificity - sensitivity down to 200 copies/mL, no peaks in 48 negative wells; 6. dUTP/UDG anti-contamination system; 7. Compatible with multiple platforms and assays - validated on mainstream real-time PCR instruments. |
| Sensitivity / LOD | 200 copies/mL; detection rate and Ct values at the limit of detection are unaffected by fast programs or 37 °C accelerated storage of the full premix. |
| Specificity | No amplification peaks in 48 negative wells; no detectable residual E. coli, Pseudomonas aeruginosa, or human genomic DNA background across 48 wells. |
| Reaction Conditions / Protocol | Conventional program: 95 °C 5 min; 40 cycles (95 °C 15 s, 60 °C 30 s) - approximately 50 min. Fast program: 95 °C 15 s; 40 cycles (95 °C 2 s, 60 °C 8 s) - approximately 20 min. Example alternative program: 95 °C 90 s; 45 cycles (95 °C 5 s, 55 °C 30 s). |
| Components / Formulation | One-tube premix containing a new-generation antibody-based hot-start Taq DNA polymerase, optimized buffer, dNTPs (with dUTP replacing dTTP), and the UDG anti-contamination system. |
| Storage Conditions | Store at -25 to -15 °C. |
| Shelf Life | 1 year when stored at -25 to -15 °C |
| Package Specifications | 100 T 1,000 T 10,000 T |
| Product Form | One-tube liquid full premix |
| Quality Control | Sensitivity and detection-rate equivalence between conventional and fast programs verified on multiplex plasmid templates; full-premix stability verified after 7 days at 37 °C with customer-supplied primer/probe sets on plasmid and real-sample viral nucleic acids; tolerance to multiple template diluents (including saline) confirmed. |
| Expiration Date / Stability | Valid for 1 year at -25 to -15 °C; full premix stable after 7 days at 37 °C and after 20 freeze-thaw cycles. |
| Compatibility | Compatible with mainstream real-time PCR instruments (96-well and 48-well formats); validated for more than 40 different multiplex target types; tolerant of various template diluents, with better saline tolerance than comparable products. |
| Application Notes / Precautions | Primers and probes can be pre-mixed with the reagent in advance and stored; performance remains stable after 7 days at 37 °C. The dUTP/UDG system effectively prevents carry-over aerosol contamination. |
For research use only, not for clinical use.
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