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| Product Name | Superpro II One-Step RT-qPCR Master Mix |
| Catalog No. | RESK-0092 |
| Description | This master mix is a one-tube, fully premixed reagent kit for single-plex or multiplex quantitative PCR using RNA as the template. Reverse transcription and quantitative PCR are performed sequentially in the same reaction tube, which simplifies handling and reduces the risk of contamination. The formulation combines a new-generation antibody-based hot-start Taq DNA polymerase, a thermostable reverse transcriptase, and a buffer system specifically optimized for one-tube, fully premixed RT-qPCR, allowing primers and probes to be pre-dispensed into the reaction mix so that only the template needs to be added at the time of use. A dUTP/UDG carryover prevention system is incorporated to effectively control aerosol-borne amplicon contamination. |
| Intended Use | Quantitative detection of RNA targets by single-plex or multiplex one-step RT-qPCR; suitable for gene expression analysis and RNA pathogen detection in research workflows. |
| Principle / Technology | One-step RT-qPCR: a thermostable reverse transcriptase first synthesizes cDNA from the RNA template, followed by real-time PCR amplification driven by an antibody-based hot-start Taq DNA polymerase in the same tube. The hot-start mechanism suppresses non-specific priming during reaction setup. A dUTP/UDG system degrades carryover PCR products from previous reactions to prevent false positives caused by aerosol contamination. |
| Detection Method | Real-time quantitative PCR (probe-based RT-qPCR, e.g., TaqMan hydrolysis probes) |
| Sample Type | RNA templates (e.g., total RNA or viral RNA, including influenza A/B RNA) |
| Performance Range / Specifications | Exceptional full-premix stability: with primers and probes pre-dispensed, performance remains stable after 5 days at 37°C High sensitivity and specificity provided by a genetically engineered antibody-based hot-start Taq DNA polymerase dUTP/UDG anti-contamination system effectively prevents aerosol-borne carryover contamination Broad platform compatibility: validated on mainstream qPCR instruments |
| Sensitivity / LOD | Reliably amplifies low-abundance templates; verified with influenza A/B templates down to 200 copies/mL (no significant Ct shift after 5 days at 37°C in fully premixed format). |
| Specificity | Antibody-mediated hot-start Taq polymerase minimizes non-specific amplification and primer-dimer formation, delivering high detection specificity. |
| Reaction Conditions / Protocol | One-tube protocol: reverse transcription and qPCR are performed in the same reaction tube. Primers and probes can be premixed into the master mix in advance; at the time of use, only the RNA template needs to be added. |
| Components / Formulation | Complete one-step RT-qPCR premix containing: Antibody-based hot-start Taq DNA polymerase Thermostable reverse transcriptase Optimized reaction buffer (Mg2+, dNTPs including dUTP) Uracil-DNA glycosylase (UDG) for carryover contamination control |
| Storage Conditions | Store at -25 to -15°C. |
| Shelf Life | 1 year when stored at -25 to -15°C. |
| Package Specifications | 50 T 100 T 1000 T 10000 T |
| Product Form | Liquid master mix (fully premixed, ready-to-use upon template addition) |
| Expiration Date / Stability | Stable for 1 year at -25 to -15°C; fully premixed reactions (with primers/probes) remain stable for 5 days at 37°C. |
| Regulatory / Compliance | For research use only. |
| Compatibility | Compatible with mainstream real-time PCR instruments, across mainstream platforms. |
| Application Notes / Precautions | Primers and probes may be pre-dispensed into the master mix; the fully assembled reaction mix retains stable performance for 5 days at 37°C. The dUTP/UDG system protects against carryover contamination; avoid introducing previously amplified PCR products into the workspace. |
For research use only, not for clinical use.
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