Superpro I Universal TaqMan Multiplex qPCR Master Mix (UDG)
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Superpro I Universal TaqMan Multiplex qPCR Master Mix (UDG)

Cat.No: RESK-0105 Datasheet

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Product Name Superpro I Universal TaqMan Multiplex qPCR Master Mix (UDG)
Catalog No. RESK-0105
Description A 2x universal TaqMan multiplex qPCR master mix built on a next-generation antibody-based hot-start Taq DNA polymerase. The premix contains the reaction buffer and enzyme blend, uses dUTP in place of dTTP, and incorporates a uracil-DNA glycosylase (UDG) carryover contamination control system to minimize aerosol-borne amplicon contamination. Dedicated enhancers suppress nonspecific amplification and boost multiplex amplification efficiency, enabling reliable up to 4-plex reactions while maintaining primer efficiency. The formulation delivers high sensitivity and strong specificity.
Intended Use Universal master mix for probe-based (TaqMan) quantitative real-time PCR, including multiplex assays of up to four targets. For research use only.
Principle / Technology Probe-based quantitative PCR using an antibody-blocked hot-start Taq DNA polymerase that is activated during the initial denaturation step. A dUTP/UDG carryover prevention system degrades uracil-containing amplicons from previous reactions before amplification.
Detection Method Real-time fluorescence quantitative PCR with hydrolysis (TaqMan) probes
Performance Range / Specifications High sensitivity and strong specificity
Supports up to 4-plex multiplex amplification
Maintains primer amplification efficiency in multiplex reactions
Specificity Antibody-mediated hot-start mechanism plus nonspecific amplification inhibitors reduce primer-dimer and off-target amplification.
Components / Formulation 2x premix containing reaction buffer, antibody hot-start Taq DNA polymerase, UDG, dNTPs (dUTP replacing dTTP), Mg2+, and multiplex amplification enhancers.
Storage Conditions Store at -25°C to -15°C.
Shelf Life 1 year when stored at -25°C to -15°C.
Package Specifications 1 mL; 5 mL; 100 mL; 5 x 1 mL; 25 mL
Product Form Liquid 2x master mix
Concentration 2x
Expiration Date / Stability Stable for 1 year at -25°C to -15°C.
Regulatory / Compliance For research use only. Not for use in diagnostic procedures.
Compatibility Compatible with mainstream real-time PCR instruments and TaqMan probe-based assays.
Application Notes / Precautions Use dUTP-containing reactions together with the built-in UDG step to control carryover contamination; avoid repeated freeze-thaw cycles.

For research use only, not for clinical use.

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