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| Product Name | Superpro I Universal TaqMan Multiplex qPCR Master Mix (UDG) |
| Catalog No. | RESK-0105 |
| Description | A 2x universal TaqMan multiplex qPCR master mix built on a next-generation antibody-based hot-start Taq DNA polymerase. The premix contains the reaction buffer and enzyme blend, uses dUTP in place of dTTP, and incorporates a uracil-DNA glycosylase (UDG) carryover contamination control system to minimize aerosol-borne amplicon contamination. Dedicated enhancers suppress nonspecific amplification and boost multiplex amplification efficiency, enabling reliable up to 4-plex reactions while maintaining primer efficiency. The formulation delivers high sensitivity and strong specificity. |
| Intended Use | Universal master mix for probe-based (TaqMan) quantitative real-time PCR, including multiplex assays of up to four targets. For research use only. |
| Principle / Technology | Probe-based quantitative PCR using an antibody-blocked hot-start Taq DNA polymerase that is activated during the initial denaturation step. A dUTP/UDG carryover prevention system degrades uracil-containing amplicons from previous reactions before amplification. |
| Detection Method | Real-time fluorescence quantitative PCR with hydrolysis (TaqMan) probes |
| Performance Range / Specifications | High sensitivity and strong specificity Supports up to 4-plex multiplex amplification Maintains primer amplification efficiency in multiplex reactions |
| Specificity | Antibody-mediated hot-start mechanism plus nonspecific amplification inhibitors reduce primer-dimer and off-target amplification. |
| Components / Formulation | 2x premix containing reaction buffer, antibody hot-start Taq DNA polymerase, UDG, dNTPs (dUTP replacing dTTP), Mg2+, and multiplex amplification enhancers. |
| Storage Conditions | Store at -25°C to -15°C. |
| Shelf Life | 1 year when stored at -25°C to -15°C. |
| Package Specifications | 1 mL; 5 mL; 100 mL; 5 x 1 mL; 25 mL |
| Product Form | Liquid 2x master mix |
| Concentration | 2x |
| Expiration Date / Stability | Stable for 1 year at -25°C to -15°C. |
| Regulatory / Compliance | For research use only. Not for use in diagnostic procedures. |
| Compatibility | Compatible with mainstream real-time PCR instruments and TaqMan probe-based assays. |
| Application Notes / Precautions | Use dUTP-containing reactions together with the built-in UDG step to control carryover contamination; avoid repeated freeze-thaw cycles. |
For research use only, not for clinical use.
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