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| Product Name | RT-LAMP Lyo-Ready Fluorescent Probe Assay Kit (Bst Plus HSS) |
| Catalog No. | RESK-0122 |
| Description | The RT-LAMP Lyo-Ready Fluorescent Probe Assay Kit (Bst Plus HSS) is a fluorescent probe-based RT-LAMP kit capable of isothermal amplification from both DNA and RNA templates. It combines a new-generation aptamer-blocked Bst Plus HSS DNA Polymerase, a heat-stable reverse transcriptase (HSS), murine RNase inhibitor, and RNase H II within a finely optimized buffer system, enabling highly efficient, rapid, and specific detection reactions, and supports reaction setup at room temperature. Both the buffer and enzyme components are glycerol-free and, together with the lyoprotectant provided, are suitable for lyophilization. |
| Intended Use | Rapid isothermal amplification and fluorescent probe-based detection of DNA and RNA targets; designed for development of lyophilized molecular diagnostic reagents. For research use. |
| Principle / Technology | Loop-mediated isothermal amplification (LAMP/RT-LAMP) driven by an aptamer-blocked Bst Plus HSS DNA Polymerase and a heat-stable reverse transcriptase. RNase H II supports the fluorescent probe detection mechanism. Glycerol-free formulation supports lyophilized product preparation. |
| Detection Method | Fluorescent probe-based detection: a target-specific fluorescent probe is cleaved during amplification (RNase H II-mediated), generating a real-time fluorescence signal monitored on a fluorescence detection instrument. |
| Sample Type | Extracted DNA or RNA from pathogen samples. |
| Performance Range / Specifications | Supports reaction setup at room temperature. Buffer and enzyme components are glycerol-free; a lyoprotectant is provided for preparation of lyophilized products. |
| Specificity | Probe-based detection with RNase H II provides high target specificity. |
| Reaction Conditions / Protocol | Isothermal amplification on a real-time fluorescence detection instrument; reaction setup can be performed at room temperature. |
| Components / Formulation | Aptamer-blocked Bst Plus HSS DNA Polymerase (glycerol-free) Heat-stable reverse transcriptase (HSS) Murine RNase inhibitor RNase H II Optimized glycerol-free reaction buffer system Lyoprotectant |
| Storage Conditions | Store at -25 °C to -15 °C. |
| Shelf Life | 1 year |
| Package Specifications | 16 T; 100 T; 1,000 T |
| Product Form | Glycerol-free liquid reagent kit, lyophilization-ready (lyoprotectant included) |
| Expiration Date / Stability | Stable for 1 year when stored at -25 °C to -15 °C. |
| Compatibility | Compatible with real-time fluorescence detection instruments. |
| Application Notes / Precautions | Reaction setup can be performed at room temperature. Buffer and enzyme components are glycerol-free and can be lyophilized using the supplied lyoprotectant. |
For research use only, not for clinical use.
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