- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | RT-LAMP Fluorescent Assay Kit (Bst Plus HSS) |
| Catalog No. | RESK-0120 |
| Description | The RT-LAMP Fluorescent Assay Kit (Bst Plus HSS) is a second-generation fluorescent dye-based RT-LAMP kit capable of isothermal amplification from both DNA and RNA templates. It combines a new-generation aptamer-blocked Bst Plus DNA Polymerase, a heat-stable reverse transcriptase (HSS), and murine RNase inhibitor within a finely optimized buffer system, enabling highly efficient, rapid, and specific detection reactions. The kit also supports reaction setup at room temperature. |
| Intended Use | Rapid isothermal amplification and fluorescent detection of DNA and RNA targets for molecular diagnostic reagent development and pathogen testing applications. For research use. |
| Principle / Technology | Loop-mediated isothermal amplification (LAMP/RT-LAMP) driven by an aptamer-blocked Bst Plus DNA Polymerase and a heat-stable reverse transcriptase, allowing DNA and RNA templates to be amplified at a constant temperature. |
| Detection Method | Fluorescent dye-based detection. An intercalating fluorescent dye (e.g., SYBR Green or Syto-type dyes) binds double-stranded DNA during amplification, increasing the fluorescence signal 800-1,000-fold; amplification is monitored in real time on a fluorescence quantitative PCR instrument. |
| Sample Type | Extracted DNA or RNA from pathogen samples (e.g., African swine fever virus nucleic acid, influenza B virus controls). |
| Performance Range / Specifications | Versatility: validated with more than 20 animal- and human-related pathogen targets with GC contents of 30%-65%, delivering rapid and consistent time-to-peak. Stability: performance is unaffected after 20 freeze-thaw cycles or 7 days of accelerated storage at 37 °C; supports reaction setup at room temperature. |
| Sensitivity / LOD | African swine fever virus (ASFV) nucleic acid extracted from real samples: down to 15 copies/reaction. Influenza B virus quality control material: down to 10 copies/reaction. Positive detection is defined as a signal peak arising within 30 min. |
| Specificity | Validated across multiple DNA and RNA targets; no non-specific amplification in no-template controls (NTC) within 60 min. |
| Reaction Conditions / Protocol | Isothermal incubation at 60-65 °C on a real-time fluorescence PCR instrument; results are typically available within approximately 30 min. Reaction setup can be performed at room temperature. |
| Components / Formulation | Aptamer-blocked Bst Plus DNA Polymerase Heat-stable reverse transcriptase (HSS) Murine RNase inhibitor Optimized reaction buffer system Fluorescent dye component (Component D) |
| Storage Conditions | Store at -25 °C to -15 °C. Component D must be protected from light. |
| Shelf Life | 1 year |
| Package Specifications | 100 T; 1,000 T |
| Product Form | Liquid reagent kit |
| Expiration Date / Stability | Stable for 1 year when stored at -25 °C to -15 °C. Performance is unaffected after 20 freeze-thaw cycles or 7 days at 37 °C (accelerated stability testing), with positive detection maintained and no non-specific amplification. |
| Compatibility | Compatible with real-time fluorescence (quantitative) PCR instruments. |
| Application Notes / Precautions | Protect Component D from light. Reaction setup can be performed at room temperature. |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
