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| Product Name | RNA Lyophilized Rapid Isothermal Amplification Kit (Basic) |
| Catalog No. | RESK-0125 |
| Description | This kit is a molecular biology reagent designed for the rapid, efficient, and specific isothermal amplification of nucleic acid fragments from RNA templates. Supplied as an in-situ lyophilized powder for maximum convenience, it combines reverse transcription with rapid isothermal nucleic acid amplification technology, enabling fast amplification of target sequences under a constant temperature of 39-42 °C. It is widely applicable in life science research, clinical diagnostics, and molecular testing. This is the basic (electrophoresis-type) format, in which amplification products are analyzed by gel electrophoresis. |
| Intended Use | Rapid isothermal amplification of RNA target sequences for life science research, clinical diagnostic development, and molecular detection applications. |
| Principle / Technology | Reverse transcription coupled with recombinase polymerase amplification (RT-RPA). At the reaction temperature, reverse transcriptase uses specific primers and the RNA template to synthesize cDNA strands. The recombinase UvsX anchors to the primers to form recombinase-primer complexes, which recognize homologous target sequences and mediate strand exchange. Single-strand binding protein then binds the displaced single-stranded DNA to form a stable D-loop structure. With the aid of the accessory factor UvsY and a crowding agent, a conformational change in the recombinase-primer complex exposes the primer 3' ends; DNA polymerase binds and initiates extension, and both upstream and downstream primers drive simultaneous amplification to generate double-stranded products. |
| Detection Method | Gel electrophoresis (basic/electrophoresis-type RT-RPA format) |
| Sample Type | RNA templates |
| Performance Range / Specifications | Lyophilized powder format for convenient use; rapid amplification of target nucleic acids at a constant temperature of 39-42 °C. |
| Reaction Conditions / Protocol | Isothermal amplification at 39-42 °C. |
| Storage Conditions | Store at -25 to -15 °C. Aliquot the reagent in advance before use. |
| Shelf Life | 1 year when stored at -25 to -15 °C |
| Package Specifications | 16 T 48 T 100 T |
| Product Form | In-situ lyophilized powder |
| Expiration Date / Stability | Valid for 1 year at -25 to -15 °C. |
For research use only, not for clinical use.
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