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| Product Name | Purepro I TaqMan qPCR Master Mix (UDG Plus) |
| Catalog No. | RESK-0090 |
| Description | This is a real-time quantitative PCR (qPCR) reagent built on a new-generation antibody-based hot-start Taq DNA polymerase. The enzyme undergoes a specific purification process that removes residual host Escherichia coli DNA as well as DNA from common environmental bacteria. The 2x TaqMan qPCR premix contains reaction buffer and an enzyme blend, already includes Mg2+ and dNTPs, and is supplemented with factors that suppress non-specific PCR amplification and enhance multiplex qPCR amplification efficiency, supporting up to 4-plex reactions while maintaining primer efficiency. The mix contains uracil-DNA glycosylase (UDG) to effectively prevent aerosol contamination. |
| Intended Use | For TaqMan probe-based real-time quantitative PCR, especially assays requiring ultra-low background of residual bacterial/host DNA, including multiplex (up to 4-plex) detection. |
| Principle / Technology | Antibody-based hot-start Taq DNA polymerase purified by a dedicated process to remove residual host (E. coli) and common environmental bacterial DNA; UDG-based carry-over contamination control; enhancers suppress non-specific amplification and improve multiplex efficiency. |
| Detection Method | Real-time fluorescence quantitative PCR (TaqMan probe-based). |
| Sample Type | DNA templates, including ultra-low-concentration samples. |
| Performance Range / Specifications | Low host DNA residue: genomic DNA residues of E. coli, Pseudomonas aeruginosa, MecA and Acinetobacter baumannii not detected across 48 wells Good stability: stable performance after 12 months at 4 °C, with negative controls remaining within the controlled range Wide linear range and high amplification efficiency: linear detection range of 10 to 1x10^5 copies/uL in the qPCR system High sensitivity: ultra-low-concentration samples (1 copy/uL) are stably detected with 100% detection rate |
| Sensitivity / LOD | 1 copy/uL stably detected; in validation testing, a 1 copy/uL template was detected in 20/20 replicates (100% detection). |
| Specificity | Contains factors that effectively suppress non-specific PCR amplification; no amplification peaks observed over 40 cycles when amplifying sterile ultrapure water with a 4-plex Pseudomonas/Acinetobacter/E. coli/mecA primer-probe panel. |
| Reaction Conditions / Protocol | Linear detection range of 10 to 1x10^5 copies/uL; standard qPCR cycling (40 cycles validated for background testing). |
| Components / Formulation | 2x premix containing reaction buffer, antibody-based hot-start Taq DNA polymerase (low-residue purified), UDG enzyme, Mg2+ and dNTPs. |
| Storage Conditions | Store at -25 to -15 °C. |
| Shelf Life | 1 year when stored at -25 to -15 °C. |
| Package Specifications | 1 mL; 5x 1 mL; 5 mL; 20 mL; 100 mL |
| Product Form | 2x liquid master mix. |
| Quality Control | Residual host DNA: no detection of E. coli, Pseudomonas aeruginosa, MecA or Acinetobacter baumannii genomic DNA across 48 wells. Stability: after 12 months at 4 °C, performance remains stable and negative controls stay within range compared with -20 °C storage. Sensitivity: 1 copy/uL template detected in 20/20 replicates. |
| Purity | Enzyme purified by a specific process to remove residual host E. coli DNA and common environmental bacterial DNA; genomic DNA of E. coli, P. aeruginosa, MecA and A. baumannii not detected in 48-well testing. |
| Expiration Date / Stability | 1 year at -25 to -15 °C; performance remains stable after 12 months at 4 °C. |
| Compatibility | Supports up to 4-plex reactions while maintaining primer efficiency; suitable for TaqMan probe-based qPCR systems. |
| Application Notes / Precautions | Contains UDG to prevent aerosol carry-over contamination. Low host DNA background makes the mix suitable for assays targeting bacterial genes (e.g., mecA) where residual DNA would cause false positives. |
For research use only, not for clinical use.
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