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| Product Name | Mycoplasma PCR Detection Kit (Inhibitor-Tolerant) |
| Catalog No. | RESK-0193 |
| Description | The Mycoplasma PCR Detection Kit (Inhibitor-Tolerant) is designed for the detection of mycoplasma in a wide range of biological products, such as cell products, animal serum and culture media. The kit uses multiple primer pairs to specifically amplify conserved regions of the mycoplasma genome by PCR, enabling accurate detection of mycoplasma. Studies show that the most frequently occurring species in mycoplasma contamination of cell cultures are M. orale, M. arginini, M. hyorhinis, A. laidlawii, M. fermentans, M. pneumoniae and M. salivarium. This kit detects more than 60 mycoplasma species, including these 7 common species. The kit offers strong specificity, high sensitivity and simple operation. Compared with conventional PCR-based mycoplasma detection kits, it is more tolerant to interfering substances in samples, effectively avoiding weak-positive and false-negative results and greatly improving the mycoplasma detection rate. The detection limit is as low as 5 copies. |
| Intended Use | Detection of mycoplasma contamination in various biological products, including cell products, animal serum and culture media. |
| Principle / Technology | Endpoint PCR using multiple primer pairs that specifically amplify conserved regions of the mycoplasma genome. |
| Detection Method | PCR amplification of conserved mycoplasma genomic regions. |
| Sample Type | Cell products, animal serum, culture media and other biological products. |
| Performance Range / Specifications | Detects more than 60 mycoplasma species, including the 7 species most common in cell culture contamination: M. orale, M. arginini, M. hyorhinis, A. laidlawii, M. fermentans, M. pneumoniae and M. salivarium. Stronger tolerance to interfering substances in samples than conventional PCR-based mycoplasma detection kits, effectively avoiding weak-positive and false-negative results and greatly improving the detection rate. |
| Sensitivity / LOD | Detection limit as low as 5 copies. |
| Specificity | Multiple primer pairs specifically amplify conserved regions of the mycoplasma genome, providing strong specificity and accurate detection. |
| Components / Formulation | The kit comprises 7 components in total, supplied as Part I and Part II. Upon receipt, verify that all 7 components are complete and immediately store each part at its indicated temperature. |
| Storage Conditions | Part I: store at room temperature. Part II: store at -25 to -15 °C. |
| Shelf Life | 2 years (Part I stored at room temperature; Part II stored at -25 to -15 °C). |
| Package Specifications | 10 T, 50 T, 100 T |
| Shipping Conditions | Part I: shipped at ambient temperature. Part II: shipped on dry ice. |
| Regulatory / Compliance | For research use only. |
| Application Notes / Precautions | The enhanced inhibitor tolerance of this kit reduces weak-positive and false-negative readouts caused by interfering substances in samples, improving the overall mycoplasma detection rate. Check that all 7 components are present upon receipt and store each part at its designated temperature immediately. |
For research use only, not for clinical use.
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