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| Product Name | Multiplex One-Step RT-PCR Kit |
| Catalog No. | RESK-0093 |
| Description | This kit is designed for multiplex PCR amplification using RNA as the template. Reverse transcription and PCR are carried out in the same reaction tube, simplifying the workflow. A thermostable reverse transcriptase efficiently synthesizes first-strand cDNA, and a hot-start Taq DNA polymerase drives the subsequent amplification. The kit contains an optimized MP Buffer and an Enzymes Mix; the buffer already includes Mg2+ and dNTPs, together with factors that effectively suppress non-specific PCR amplification and enhance multiplex amplification efficiency, enabling reliable amplification of up to eight targets in a single reaction while maintaining primer amplification efficiency. |
| Intended Use | Multiplex amplification of RNA targets in a single reaction tube; suitable for multi-target RNA analysis and pathogen detection research. |
| Principle / Technology | One-step RT-PCR: a thermostable reverse transcriptase converts RNA into first-strand cDNA, which is then amplified in the same tube by a hot-start Taq DNA polymerase. The optimized buffer contains additives that suppress non-specific amplification and improve multiplex amplification efficiency, supporting up to 8-plex reactions. |
| Detection Method | Multiplex one-step RT-PCR (one-tube reverse transcription followed by PCR amplification) |
| Sample Type | RNA templates |
| Performance Range / Specifications | Reverse transcription and PCR performed in the same reaction tube for simple operation; supports multiplex amplification of up to 8 targets in one reaction. |
| Specificity | The buffer contains factors that effectively inhibit non-specific PCR amplification, preserving high primer amplification efficiency in multiplex reactions. |
| Reaction Conditions / Protocol | One-tube protocol: reverse transcription and PCR amplification are performed sequentially in the same reaction tube without intermediate handling. |
| Components / Formulation | Optimized MP Buffer (contains Mg2+ and dNTPs, plus factors that suppress non-specific amplification and enhance multiplex efficiency) Enzymes Mix (thermostable reverse transcriptase and hot-start Taq DNA polymerase) |
| Storage Conditions | Store at -25 to -15°C. |
| Shelf Life | 1 year when stored at -25 to -15°C. |
| Package Specifications | 100 T 50 T |
| Product Form | Liquid kit (buffer and enzyme mix) |
| Expiration Date / Stability | Stable for 1 year at -25 to -15°C. |
| Regulatory / Compliance | For research use only. |
| Compatibility | Supports up to 8-plex amplification in a single reaction. |
For research use only, not for clinical use.
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