Monkeypox Virus Real-Time qPCR Kit
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Monkeypox Virus Real-Time qPCR Kit

Cat.No: RESK-0106 Datasheet

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Product Details Related Products
Product Name Monkeypox Virus Real-Time qPCR Kit
Catalog No. RESK-0106
Description A semi-finished real-time qPCR kit for the detection of monkeypox virus (MPXV) DNA. The kit includes monkeypox virus-specific primers and probes, a 2x premix based on a next-generation antibody-based hot-start Taq DNA polymerase (with Mg2+ and dNTPs), and a positive control. Factors that suppress nonspecific amplification and enhance multiplex amplification efficiency are included to improve overall amplification efficiency and promote effective amplification of low-concentration templates. The kit contains all components required for qPCR except the template, making it convenient to use.
Intended Use Semi-finished research reagent kit for qualitative detection of monkeypox virus (MPXV) nucleic acid by real-time qPCR. For research use only; not for clinical diagnostic use.
Principle / Technology TaqMan probe-based real-time PCR: virus-specific primers and a fluorescently labeled probe amplify and detect MPXV target DNA, with an antibody-based hot-start Taq polymerase ensuring specific amplification.
Detection Method Real-time fluorescence quantitative PCR (TaqMan probe)
Sample Type Extracted nucleic acid (DNA) samples containing potential monkeypox virus target sequences
Performance Range / Specifications Contains all qPCR components except the template; easy to use
Supports efficient amplification of low-concentration templates
Sensitivity / LOD Formulated to support effective amplification of low-concentration templates (enhanced amplification efficiency for low-copy targets).
Specificity Monkeypox virus-specific primer/probe set combined with antibody hot-start Taq and nonspecific amplification inhibitors.
Components / Formulation Monkeypox virus primer/probe mix
2x qPCR premix with antibody hot-start Taq DNA polymerase, Mg2+ and dNTPs
Positive control
Storage Conditions Store at -25°C to -15°C.
Shelf Life 1 year when stored at -25°C to -15°C.
Package Specifications 24 T; 48 T; 96 T
Product Form Liquid reagent kit (semi-finished)
Quality Control Positive control included for assay performance verification.
Concentration 2x premix
Expiration Date / Stability Stable for 1 year at -25°C to -15°C.
Regulatory / Compliance For research use only. Not for use in diagnostic procedures.
Compatibility Compatible with mainstream real-time PCR instruments supporting TaqMan probe chemistry.
Application Notes / Precautions For research use only. Template nucleic acid is not included and must be extracted separately. Avoid repeated freeze-thaw cycles.

For research use only, not for clinical use.

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