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| Product Name | MEM Non-Essential Amino Acids, 100x Solution, Liquid, Sterile, Cell Culture Grade |
| Catalog No. | ATR-CCR-0043 |
| Description | 100x concentrated MEM Non-Essential Amino Acids (NEAA) solution containing seven amino acids: L-alanine, L-asparagine, L-aspartic acid, L-glutamic acid, glycine, L-proline, and L-serine at concentrations optimized for cell culture supplementation. These amino acids are classified as non-essential because many mammalian cells can synthesize them endogenously; however, supplementation is beneficial for: cell lines with high metabolic demand that outpace endogenous biosynthesis; some hybridoma, lymphocyte, and fastidious cell lines; maintaining pluripotency in embryonic stem cells; and enhancing growth of primary cells. The 100x formulation allows convenient supplementation at 1:100 dilution (e.g., 5 mL per 500 mL medium). The solution is sterile-filtered (0.1 um), endotoxin-tested, and mycoplasma-tested. |
| Intended Use | Amino acid supplementation for: cell culture media (DMEM, RPMI 1640, MEM, IMDM, Ham's F-12) to enhance growth of fastidious cell lines; hybridoma culture for monoclonal antibody production; embryonic stem cell (ESC) and induced pluripotent stem cell (iPSC) culture (NEAA supplementation is standard for mouse ESC medium); primary cell culture; lymphocyte and macrophage culture; and custom medium formulation requiring non-essential amino acid supplementation. |
| Principle / Technology | Non-essential amino acids serve as: (1) protein synthesis substrates during rapid proliferation when endogenous synthesis is rate-limiting; (2) metabolic intermediates (alanine and aspartate for gluconeogenesis; glutamate for TCA cycle; glycine for purine and glutathione synthesis; serine for one-carbon metabolism and nucleotide synthesis); (3) osmolytes and pH regulators; (4) signaling molecules (glycine as neurotransmitter in neural cultures; serine in mTOR signaling). Supplementation ensures these amino acids are not growth-limiting during high-density and rapid-growth phases of cell culture. |
| Detection Method | Thaw 100x NEAA at 2-8 C or RT. Add aseptically to sterile complete medium at 1:100 dilution (e.g., 5 mL per 500 mL medium). Mix thoroughly. Use complete medium within typical shelf-life (2-4 weeks at 2-8 C). Not typically used at higher concentrations; 1x final is standard. |
| Sample Type | Liquid supplement; ready-to-use after thawing. |
| Performance Range / Specifications | Amino acid concentrations in 100x solution: L-alanine 890 mg/dL (10 mM), L-asparagine monohydrate 1,500 mg/dL (10 mM), L-aspartic acid 1,330 mg/dL (10 mM), L-glutamic acid 1,470 mg/dL (10 mM), glycine 750 mg/dL (10 mM), L-proline 1,150 mg/dL (10 mM), L-serine 1,050 mg/dL (10 mM); each amino acid at 0.1 mM final (1:100 dilution); sterility: negative; endotoxin: <0.25 EU/mL; mycoplasma: negative. |
| Sensitivity / LOD | Not applicable — cell culture supplement. |
| Specificity | Seven non-essential amino acids at defined concentrations; no other amino acids or growth factors present. |
| Reaction Conditions / Protocol | Stable at 2-8 C for 18 months; degradation: glutamine (if present in other supplements) and tryptophan are most labile — neither is in this NEAA formulation, making it relatively stable; protect from light (riboflavin-mediated photooxidation of amino acids, especially tryptophan — not in NEAA — but other amino acids may be affected). |
| Components / Formulation | MEM Non-Essential Amino Acids, 100x Solution: L-alanine (10 mM), L-asparagine·H2O (10 mM), L-aspartic acid (10 mM), L-glutamic acid (10 mM), glycine (10 mM), L-proline (10 mM), L-serine (10 mM) in WFI quality water; 0.1 um sterile-filtered; 100 mL bottle. |
| Storage Conditions | Store at 2-8 C; do not freeze (amino acid precipitation may occur); protect from light. |
| Shelf Life | 18 months from manufacture at 2-8 C unopened; after opening: 4-6 weeks at 2-8 C with aseptic technique. |
| Package Specifications | 100 mL sterile bottle. |
| Product Form | Clear, colorless sterile liquid; no phenol red; no particulates. |
| Quality Control | Each lot: individual amino acid concentrations within +/-10% of specification (HPLC amino acid analysis); pH; osmolality; sterility (negative, USP <71>); mycoplasma (negative); endotoxin (<0.25 EU/mL); cell growth: mouse ES cells (with NEAA) maintain typical colony morphology and alkaline phosphatase expression; hybridoma growth at >=90% of reference medium. |
| Key Features | 100x MEM non-essential amino acids; 7 amino acids at defined concentrations; sterile 0.1 um filtered; cell culture grade; endotoxin <0.25 EU/mL; mycoplasma tested; essential for mESC/iPSC culture; 18-month shelf life. |
| Purity | Each amino acid: >99% pure (USP or cell culture grade); water: WFI quality; no preservatives; no animal-derived components. |
| Concentration | 100x concentrate; each amino acid at 10 mM in stock, 0.1 mM final after 1:100 dilution in medium. |
| Activity / Unit Definition | Growth promotion verified with mouse ES cells and hybridoma cell lines; NEAA-supplemented cultures outperform non-supplemented controls. |
| Molecular Weight | Glycine: 75.07 g/mol (smallest); L-glutamic acid: 147.13 g/mol (largest); L-asparagine monohydrate: 150.13 g/mol. |
| Source / Origin | All amino acids: fermentation-derived or synthetic; water: WFI quality; no animal-derived components; manufactured in ISO-certified facility. |
| pH Range / Optimal pH | pH of 100x solution: 5.0-7.0; after 1:100 dilution into medium, final pH unchanged. |
| Shipping Conditions | Cold pack (2-8 C); ambient temperature for short transit (stable at RT for 1 week). |
| Expiration Date / Stability | 18 months at 2-8 C; after opening: 4-6 weeks at 2-8 C. |
| Regulatory / Compliance | For research use only; not for diagnostic or therapeutic use. Not for human injection. Cell culture grade — not GMP-certified. |
| Compatibility | Compatible with all standard basal media (DMEM, RPMI 1640, MEM, IMDM, Ham's F-12, DMEM/F12, Neurobasal). DMEM and RPMI 1640 do not contain NEAA in their standard formulations — supplementation is common. MEM-alpha and IMDM already contain NEAA — additional supplementation may not be needed. NEAA supplementation is standard for: mouse embryonic stem cell (mESC) medium (with LIF, 15% FBS), mouse iPSC medium, hybridoma medium, and many primary cell culture protocols. The 1x NEAA concentration adds 0.1 mM of each amino acid, which is relatively low compared to essential amino acid concentrations (0.2-4 mM) and is unlikely to cause toxicity. |
| Recommended Buffer System | No buffer system — amino acids dissolved in WFI water; pH adjusted for amino acid stability. |
| Application Notes / Precautions | NEAA 100x solution may form a slight precipitate at 2-8 C, particularly L-tyrosine (not in NEAA — not a concern) and L-aspartic acid near its solubility limit. If precipitate is observed, warm to 37 C and swirl gently until dissolved before use. This supplement does not contain L-glutamine — glutamine must be supplemented separately. For mESC culture, the standard medium formulation includes: DMEM (high glucose) + 15% FBS + 1x NEAA + 1x GlutaMAX or 2 mM L-glutamine + 0.1 mM 2-mercaptoethanol + 1,000 U/mL LIF. Do not freeze this solution — freezing may cause amino acid precipitation that may not redissolve. Aliquot using aseptic technique in a biosafety cabinet. Supplement medium with NEAA just before use if medium has been stored for extended periods (>2 weeks). |
| Batch-to-Batch Consistency | Amino acid concentrations within +/-10% of specification; sterility and mycoplasma negative; cell growth >=90% of reference medium. |
For research use only, not for clinical use.
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