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| Product Name | CT26 Human GPRC5D Stable Cell Line |
| Catalog No. | ATR-CCR-0076 |
| Description | A cryopreserved, adherent CT26 cell line stably expressing tag-free human GPRC5D. The model is maintained under puromycin selection for cell-based target-binding studies. |
| Intended Use | For FACS binding assays, cell-based ELISA and other target-specific cell research applications. |
| Principle / Technology | Stable transfection followed by antibiotic selection; GPRC5D expression is qualified by flow cytometry. |
| Detection Method | Flow cytometry; cell-based ELISA |
| Sample Type | Stable mammalian cell line |
| Performance Range / Specifications | Approximately 5 x 10^6 cells in 1 mL per vial; adherent growth. |
| Specificity | Human GPRC5D |
| Reaction Conditions / Protocol | Culture in RPMI 1640 with 10% FBS and 8 ug/mL puromycin. |
| Components / Formulation | Cryopreservation medium: 35% FBS, 55% RPMI 1640 and 10% DMSO. |
| Storage Conditions | Store frozen in liquid nitrogen or at -80 C. |
| Package Specifications | 1 vial; approximately 5 x 10^6 cells in 1 mL |
| Product Form | Cryopreserved stable cell line |
| Quality Control | Target expression verified by staining with an anti-GPRC5D antibody at 2 ug per 1 x 10^6 cells, followed by a FITC-conjugated secondary antibody and flow-cytometric analysis. |
| Key Features | Stable human GPRC5D expression; adherent growth; puromycin resistance; tag-free; qualified for FACS and cell-based ELISA applications. |
| Source / Origin | CT26 cells stably expressing human GPRC5D. |
| Regulatory / Compliance | For research use only; not for clinical diagnosis or treatment. |
| Compatibility | FACS binding assays; cell-based ELISA |
| Recommended Buffer System | RPMI 1640 with 10% FBS and 8 ug/mL puromycin |
| Application Notes / Precautions | Maintain puromycin selection during routine culture. Keep the vial frozen until cell recovery and use aseptic cell-culture technique. |
| Genotype | Stable human GPRC5D expression; puromycin-resistant. |
| Alternative Names / Synonyms | GPRC5D; G-protein coupled receptor family C group 5 member D |
| Lead Time | 1-3 working days |
| Host Species | Human |
| Tag Information | Tag-Free |
| UniProt Accession | Q9NZD1 |
For research use only, not for clinical use.
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