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| Product Name | Cell Freezing Medium (CryoStor CS10) |
| Catalog No. | ATR-CCR-0027 |
| Description | A defined, serum-free, and animal-component-free cryopreservation medium formulated with 10% dimethyl sulfoxide for the cryopreservation of mammalian cells. The medium is optimized for post-thaw cell viability and recovery across a broad range of cell types. |
| Intended Use | Cryopreservation of mammalian cells, including primary cells, stem cells, and established cell lines, in liquid nitrogen vapor phase or mechanical -150°C freezers. |
| Principle / Technology | The optimized formulation balances cryoprotectant penetration, osmotic buffering, and oxidative stress protection during freeze-thaw cycles, minimizing ice crystal damage and apoptosis. |
| Detection Method | Post-thaw viability assessed by trypan blue exclusion, flow cytometry with viability dyes, or cell attachment and proliferation assays |
| Sample Type | Suspension or adherent mammalian cells harvested by trypsinization or centrifugation and resuspended as a single-cell suspension |
| Performance Range / Specifications | Recommended freezing density: 1–10 × 10^6 cells/mL depending on cell type; cooling rate: approximately -1°C/min in a controlled-rate freezer or Mr. Frosty container; storage: ≤-150°C (liquid nitrogen vapor phase) |
| Sensitivity / LOD | Post-thaw viability typically >80% for most cell lines; >90% for robust cell types such as HEK293, CHO, and Jurkat |
| Specificity | Formulation optimized for broad mammalian cell compatibility including primary, stem, and established cell lines; animal-component-free composition eliminates risks of adventitious agent introduction |
| Reaction Conditions / Protocol | Harvest cells and count, centrifuge at 300g for 5 minutes, resuspend cell pellet in cold CryoStor CS10 at the recommended density, transfer 1 mL aliquots to cryovials, place vials in a controlled-rate freezing container, transfer to -80°C for 4–24 hours, then move to liquid nitrogen vapor phase for long-term storage |
| Components / Formulation | CryoStor CS10 cryopreservation medium, sterile and ready to use; pre-formulated with 10% DMSO, optimized electrolyte composition, and antioxidant supplements |
| Storage Conditions | -20°C protected from light; can be stored at 2–8°C for up to 2 weeks; thaw at 2–8°C or room temperature before use |
| Shelf Life | 24 months at -20°C |
| Package Specifications | 100 mL |
| Product Form | Sterile, ready-to-use liquid in PETG bottle |
| Quality Control | Each lot tested for sterility, endotoxin (<0.5 EU/mL), pH, osmolality, and post-thaw viability using a standardized Jurkat cell freezing and recovery assay (>80% viable recovery required for lot release) |
| Key Features | Defined, serum-free formulation ensures lot-to-lot reproducibility; animal-component-free composition supports regulatory compliance for cell therapy applications; optimized antioxidant formulation reduces post-thaw apoptosis |
For research use only, not for clinical use.
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