- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | Poly-L-Lysine Coating Solution, 0.1 mg/mL, Sterile |
| Catalog No. | ATR-CCR-0036 |
| Description | A sterile poly-L-lysine solution at 0.1 mg/mL for coating tissue culture surfaces to enhance cell adhesion. Poly-L-lysine is a synthetic positively charged polymer that adsorbs to negatively charged culture surface, increasing the density of cell attachment sites through electrostatic interaction with the negatively charged cell membrane. This coating is particularly useful for primary neurons, transfected cell lines, and other weakly adherent cells. |
| Intended Use | Enhancement of cell adhesion to tissue culture plastic and glass surfaces for primary neurons and poorly attaching cell lines. |
| Principle / Technology | Electrostatic coating of culture surface with polycationic poly-L-lysine polymer |
| Detection Method | Phase contrast microscopy for cell attachment and spreading assessment |
| Sample Type | Primary neurons, transfected HEK293 cells, weakly adherent cell lines, and cells cultured in serum-free conditions |
| Performance Range / Specifications | Coating concentration: 0.1 mg/mL; molecular weight: 70,000–150,000 Da; coating volume: 0.5 mL per cm² |
| Sensitivity / LOD | Complete surface coverage confirmed by uniform cell attachment pattern across coated surface |
| Specificity | Poly-L-lysine enhances adhesion of all negatively charged cell types; biodegradable and non-cytotoxic |
| Reaction Conditions / Protocol | Add solution to culture vessel to cover surface; incubate 1 hr at room temperature or overnight at 4 °C; aspirate excess; wash 2× with sterile water; air dry in biosafety cabinet before seeding cells |
| Components / Formulation | Poly-L-lysine hydrobromide (0.1 mg/mL) in sterile water, 0.22 µm filtered |
| Storage Conditions | 2–8 °C; do not freeze |
| Shelf Life | 12 months at 2–8 °C |
| Package Specifications | 50 mL |
| Product Form | Liquid, sterile, ready-to-use |
| Quality Control | Cell adhesion performance verified on primary rat hippocampal neuron culture; sterility per USP <71> |
| Key Features | Ready-to-use formulation eliminates weighing and sterile-filtering steps required with lyophilized poly-lysine preparations |
| Purity | Sterile; endotoxin ≤0.25 EU/mL; mycoplasma-free |
| Concentration | As specified (1× ready-to-use or 100× concentrate) |
| Activity / Unit Definition | Cell growth promotion validated per lot on reference cell lines |
| Molecular Weight | Not applicable for basal media; varies for supplements |
| Source / Origin | Synthetic amino acids, vitamins, and inorganic salts; animal-origin components irradiated or tested |
| pH Range / Optimal pH | pH 7.2–7.6 at physiological CO₂ |
| Shipping Conditions | Ambient temperature for dry powder; cold packs for liquid media |
| Expiration Date / Stability | 12 months for liquid media; 24–36 months for dry powder |
| Regulatory / Compliance | Research use; GMP-grade options available; Certificate of Origin for animal-derived components |
| Compatibility | Compatible with standard mammalian cell culture incubator conditions (37 °C, 5% CO₂) |
| Recommended Buffer System | Sodium bicarbonate/CO₂ buffering system; HEPES-buffered options available |
| Application Notes / Precautions | Pre-warm to 37 °C before use; supplement with serum and antibiotics as needed; use aseptic technique |
| Batch-to-Batch Consistency | Osmolality within ±5% of reference; cell growth rate within ±15% of reference lot |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
