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| Product Name | Matrigel Basement Membrane Matrix, Growth Factor Reduced |
| Catalog No. | ATR-CCR-0020 |
| Description | A solubilized basement membrane preparation extracted from Engelbreth-Holm-Swarm mouse sarcoma, with reduced growth factor content, that polymerizes at 37°C to form a reconstituted basement membrane for three-dimensional cell culture. |
| Intended Use | Three-dimensional culture of cells in a physiologically relevant extracellular matrix environment; angiogenesis assays; stem cell differentiation; tumor cell invasion studies; organoid culture establishment. |
| Principle / Technology | The matrix contains laminin, collagen IV, entactin, and heparan sulfate proteoglycans that self-assemble at 37°C into a hydrogel resembling native basement membrane; growth factor reduced formulation provides a more defined environment with lower background signaling. |
| Detection Method | Tube formation assay for endothelial cells, organoid formation efficiency, cell invasion through Matrigel-coated Transwell inserts |
| Sample Type | Endothelial cells (HUVEC) for angiogenesis, tumor cells for invasion assays, stem cells for organoid culture, primary tissue-derived cells |
| Performance Range / Specifications | Gels at 37°C within 30 minutes; protein concentration 8–12 mg/mL; endotoxin <1.5 EU/mL |
| Sensitivity / LOD | N/A |
| Specificity | N/A |
| Reaction Conditions / Protocol | Thaw overnight at 4°C on ice; keep all materials and Matrigel on ice to prevent premature gelation; add to pre-chilled cultureware; incubate at 37°C for 30 min to gel. |
| Components / Formulation | Solubilized EHS mouse sarcoma basement membrane proteins in DMEM, growth factor reduced by ammonium sulfate precipitation |
| Storage Conditions | -20°C or -80°C; thaw overnight at 4°C; do not refreeze |
| Shelf Life | 12 months at -20°C |
| Package Specifications | 5 mL, 10 mL, 1.5 mL aliquot pack |
| Product Form | Frozen liquid, gels at 22–37°C |
| Quality Control | Protein concentration, gelation verification, endotoxin, sterility, angiogenesis assay functional testing |
| Key Features | Growth factor reduced; self-polymerizes at 37°C; standard for 3D culture and angiogenesis; mouse origin; widely cited in literature |
For research use only, not for clinical use.
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