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| Product Name | Opti-MEM I Reduced-Serum Medium, with GlutaMAX |
| Catalog No. | ATR-CCR-0005 |
| Description | A chemically defined reduced-serum medium formulation that supports growth of a variety of cell types at significantly lower serum concentrations, specifically optimized for cationic lipid-mediated transfection procedures. |
| Intended Use | Serum-reduced culture for transfection experiments using cationic lipid reagents; compatible with Lipofectamine and other lipid-based nucleic acid delivery systems where serum proteins can interfere with complex formation. |
| Principle / Technology | GlutaMAX dipeptide (L-alanyl-L-glutamine) provides stable glutamine source without toxic ammonia accumulation; insulin, transferrin, and defined supplements replace serum growth factors; reduced protein content minimizes interference with lipid-nucleic acid complexes. |
| Detection Method | Transfection efficiency with reporter plasmid, cell viability post-transfection, growth rate in reduced serum |
| Sample Type | HEK293, HeLa, COS-7, CHO, and other commonly transfected adherent cell lines |
| Performance Range / Specifications | Supports transfection with 50–90% efficiency depending on cell line; serum reduction to 1–5% FBS |
| Sensitivity / LOD | N/A |
| Specificity | N/A |
| Reaction Conditions / Protocol | Use unsupplemented for transfection complex formation; post-transfection, add FBS to 5% for recovery; change to complete growth medium after 4–6 hours. |
| Components / Formulation | Opti-MEM formulation with GlutaMAX-I, HEPES, sodium bicarbonate, insulin, transferrin, hypoxanthine, thymidine, trace elements, phenol red |
| Storage Conditions | 2–8°C, protect from light |
| Shelf Life | 12 months |
| Package Specifications | 500 mL, 1 L |
| Product Form | Liquid, sterile-filtered |
| Quality Control | Sterility, endotoxin, pH, osmolality, transfection compatibility validation, growth promotion |
| Key Features | Optimized for transfection; GlutaMAX dipeptide; reduced serum requirement; HEPES buffer; defined formulation; compatible with major transfection reagents |
For research use only, not for clinical use.
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