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| Product Name | Chemically Defined Lipid Concentrate, Serum-Free Medium Supplement |
| Catalog No. | ATR-CCR-0021 |
| Description | A sterile 100x chemically defined lipid emulsion supplement containing cholesterol, fatty acids, and tocopherol for the serum-free culture of cells with lipid-dependent growth requirements. |
| Intended Use | Supplementing serum-free and chemically defined cell culture media with essential lipids for the growth of CHO cells, hybridomas, insect cells, and other lipid-auxotrophic cell lines in protein production applications. |
| Principle / Technology | Lipids serve as membrane components, energy substrates, and signaling molecules; chemically defined formulation eliminates the variability and regulatory concerns of animal-derived serum lipid sources. |
| Detection Method | Cell growth in serum-free conditions, recombinant protein productivity comparison with serum-containing controls |
| Sample Type | CHO cells, hybridomas, NS0, Sp2/0, HEK293, Sf9 and High Five insect cells |
| Performance Range / Specifications | 1:100 dilution provides cholesterol, arachidonic acid, linoleic acid, linolenic acid, myristic acid, oleic acid, palmitic acid, stearic acid, and alpha-tocopherol at defined concentrations |
| Sensitivity / LOD | N/A |
| Specificity | N/A |
| Reaction Conditions / Protocol | Add 1 mL per 100 mL medium (1:100); slowly add to medium with gentle swirling to ensure proper emulsification; protect from light. |
| Components / Formulation | Arachidonic acid, Cholesterol, DL-alpha-Tocopherol Acetate, Linoleic Acid, Linolenic Acid, Myristic Acid, Oleic Acid, Palmitic Acid, Pluronic F-68, Stearic Acid, Tween 80, in cell culture grade water |
| Storage Conditions | 2–8°C, protect from light, do not freeze |
| Shelf Life | 12 months |
| Package Specifications | 100 mL, 500 mL |
| Product Form | Sterile emulsion (100x) |
| Quality Control | Lipid concentration by GC-FID, sterility, endotoxin, CHO cell growth promotion in serum-free medium |
| Key Features | Chemically defined; 100x concentrate; supports serum-free culture; animal component free; validated for CHO and hybridoma protein production |
For research use only, not for clinical use.
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