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| Product Name | Lactate-Glucose Co-Assay Kit |
| Catalog No. | BSTR-0008 |
| Description | This co-assay kit can simultaneously detect glucose and lactate in biological samples, simplifying experimental operations and reducing sample usage. It has high precision and low interference, suitable for glycolysis and energy metabolism research. |
| Test Target | Glucose (Glu) and Lactate (Lac) |
| Methodology | Enzymatic colorimetric (Glucose Oxidase, Lactate Dehydrogenase, Peroxidase) |
| Measurement Range | Glu: 0.1-25 mmol/L; Lac: 0.1-20 mmol/L |
| Sample Type | Serum, plasma, cell lysates, tissue homogenates, cell culture medium |
| Assay Time | 20 min at 37°C (10 min for each target) |
| Wavelength | Glu: 505 nm; Lac: 490 nm |
| Linearity | Glu: 0.1-25 mmol/L (R²≥0.995); Lac: 0.1-20 mmol/L (R²≥0.995) |
| Precision (Intra-assay CV) | Glu: ≤2%; Lac: ≤2% |
| Precision (Inter-assay CV) | Glu: ≤3%; Lac: ≤3% |
| Stability (Unopened) | 18 months at 2-8°C |
| Stability (Opened) | 30 days at 2-8°C |
| Stability (Reconstituted) | 7 days at 2-8°C |
| Interference (Hemoglobin) | ≤500 mg/dL (no significant interference for both) |
| Interference (Ascorbic Acid) | ≤40 mg/dL (no significant interference for both) |
| Interference (Uric Acid) | ≤80 mg/dL (no significant interference for both) |
| Reagent Composition | Glucose oxidase, lactate dehydrogenase, peroxidase, 4-aminoantipyrine, phenol, buffer |
| Reagent Form | Liquid |
| Reagent Volume | R1: 60 mL×2; R2: 20 mL×2; R3: 10 mL×2 |
| Calibration Type | External calibration |
| Calibration Levels | 4 levels for each target (0, 5, 15, 25 mmol/L for Glu; 0, 5, 15, 20 mmol/L for Lac) |
| Incubation Temperature | 37°C |
| Detection Instrument | Microplate reader, spectrophotometer |
| Application | Simultaneous quantitative determination of glucose and lactate in biological samples for research on glycolysis, energy metabolism, and metabolic disorders |
| Cross - Reactivity | No cross-reactivity between glucose and lactate detection systems |
| pH Range | 6.8-7.2 |
| Shelf Life | 18 months |
| Storage Condition | Store at 2-8°C, protected from light; avoid freezing |
| Minimum Detection Limit | Glu: 0.05 mmol/L; Lac: 0.05 mmol/L |
| Assay Format | 96-well microplate |
For research use only, not for clinical use.
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