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| Product Name | Glucose Content Assay Kit (Tissue / Bacteria / Cell) |
| Catalog No. | BSTR-0005 |
| Description | This glucose content assay kit is specially designed for detecting glucose in tissues, bacteria, and cells. It includes extraction buffer for sample preparation, has high sensitivity and low interference, suitable for in-depth research on intracellular glucose metabolism. |
| Test Target | Glucose (Glu) |
| Methodology | Enzymatic colorimetric (Glucose Oxidase, Peroxidase) |
| Measurement Range | 0.05-5 mmol/L |
| Sample Type | Tissue homogenates, bacterial lysates, cell lysates, cell culture medium |
| Assay Time | 15 min at 37°C |
| Wavelength | 505 nm |
| Linearity | 0.05-5 mmol/L (R²≥0.997) |
| Precision (Intra-assay CV) | ≤2% |
| Precision (Inter-assay CV) | ≤3% |
| Stability (Unopened) | 18 months at 2-8°C |
| Stability (Opened) | 30 days at 2-8°C |
| Stability (Reconstituted) | 7 days at 2-8°C |
| Interference (Hemoglobin) | ≤400 mg/dL (no significant interference) |
| Interference (Ascorbic Acid) | ≤40 mg/dL (no significant interference) |
| Interference (Uric Acid) | ≤80 mg/dL (no significant interference) |
| Reagent Composition | Glucose oxidase, peroxidase, 4-aminoantipyrine, phenol, glucose standard, extraction buffer |
| Reagent Form | Liquid + powder |
| Reagent Volume | Reagent 1: 10mL; Reagent 2: 10mL; Glucose standard: 10mL |
| Calibration Type | External calibration |
| Calibration Levels | 4 levels (0, 0.5, 2, 5 mmol/L) |
| Incubation Temperature | 37°C |
| Detection Instrument | Microplate reader, spectrophotometer |
| Application | Quantitative determination of glucose content in tissues, bacteria, and cells for research on intracellular glucose metabolism, energy supply, and microbial glucose utilization |
| Cross - Reactivity | No significant cross-reactivity with common intracellular substances |
| pH Range | 6.8-7.2 |
| Shelf Life | 18 months |
| Storage Condition | Store at 2-8°C, protected from light; extraction buffer can be stored at -20°C for long-term use |
| Minimum Detection Limit | 0.01 mmol/L |
| Assay Format | 96-well microplate |
For research use only, not for clinical use.
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