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Glucose Assay Kit (Glucose Oxidase Method)

Cat.No: BSTR-0013 Datasheet

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Product Name Glucose Assay Kit (Glucose Oxidase Method)
Catalog No. BSTR-0013
Description This glucose assay kit uses the glucose oxidase method for accurate quantitative detection of glucose in various biological samples. It has a wide linear range, high precision, and low interference from common substances, making it ideal for glucose metabolism research and related experimental studies.
Test Target Glucose (Glu)
Methodology Enzymatic colorimetric (Glucose Oxidase, Peroxidase)
Measurement Range 0.1-30 mmol/L
Sample Type Serum, plasma, cell lysates, tissue homogenates, cell culture medium
Assay Time 15 min at 37°C
Wavelength 505 nm
Linearity 0.1-30 mmol/L (R²≥0.995)
Precision (Intra-assay CV) ≤2%
Precision (Inter-assay CV) ≤3%
Stability (Unopened) 18 months at 2-8°C
Stability (Opened) 30 days at 2-8°C
Stability (Reconstituted) 7 days at 2-8°C
Interference (Hemoglobin) ≤500 mg/dL (no significant interference)
Interference (Ascorbic Acid) ≤50 mg/dL (no significant interference)
Interference (Uric Acid) ≤100 mg/dL (no significant interference)
Reagent Composition Glucose oxidase, peroxidase, 4-aminoantipyrine, phenol, buffer
Reagent Form Liquid
Reagent Volume R1: 60 mL×4; R2: 20 mL×4
Calibration Type External calibration
Calibration Levels 5 levels (0, 5, 10, 20, 30 mmol/L)
Incubation Temperature 37°C
Detection Instrument Microplate reader, spectrophotometer
Application Quantitative determination of glucose in biological samples for research on glucose metabolism, energy homeostasis, and metabolic disorders
Cross - Reactivity No significant cross-reactivity with maltose, lactose, fructose
pH Range 6.8-7.2
Shelf Life 18 months
Storage Condition Store unopened at 2-8°C, protected from light; opened and reconstituted reagents at 2-8°C
Minimum Detection Limit 0.05 mmol/L
Assay Format 96-well microplate / test tube

For research use only, not for clinical use.

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