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| Product Name | Fast Multiplex One-Step RT-qPCR Probe Kit (UDG Plus) |
| Catalog No. | RESK-0101 |
| Description | The Fast Multiplex One-Step RT-qPCR Probe Kit (UDG Plus) is designed for multiplex quantitative PCR using RNA templates. Reverse transcription and quantitative PCR are performed in the same reaction tube, simplifying the workflow, while the included UDG enzyme effectively prevents the risk of aerosol contamination. A thermostable reverse transcriptase efficiently synthesizes first-strand cDNA, and an antibody-based hot-start Taq DNA polymerase drives quantitative amplification. The kit contains an optimized MP buffer and an enzyme mix; the buffer already includes Mg2+ and dNTPs plus factors that suppress non-specific amplification and enhance multiplex amplification efficiency, enabling up to 4-plex reactions while maintaining primer efficiency. The fast formulation is optimized for rapid cycling programs. |
| Intended Use | Rapid multiplex one-step RT-qPCR detection of RNA targets for molecular diagnostic reagent development and research applications. |
| Principle / Technology | One-step RT-qPCR: reverse transcription and quantitative PCR are performed sequentially in the same reaction tube. A thermostable reverse transcriptase efficiently synthesizes first-strand cDNA, followed by quantitative amplification with an antibody-based hot-start Taq DNA polymerase. The incorporated dUTP/UDG anti-contamination system degrades uracil-bearing carryover amplicons, effectively preventing aerosol contamination and reducing false positives. |
| Detection Method | TaqMan probe-based one-step real-time fluorescence RT-qPCR. |
| Sample Type | RNA templates. |
| Performance Range / Specifications | Supports up to 4-plex one-step RT-qPCR reactions while maintaining primer amplification efficiency. Fast formulation optimized for rapid cycling programs. |
| Specificity | The buffer contains factors that effectively suppress non-specific PCR amplification and factors that enhance amplification efficiency in multiplex qPCR, ensuring primer efficiency in up to 4-plex reactions. |
| Reaction Conditions / Protocol | One-step protocol: reverse transcription and quantitative PCR are carried out in the same tube for simple operation; optimized for fast cycling programs. |
| Components / Formulation | Optimized MP buffer (contains Mg2+ and dNTPs, with dUTP/UDG system) Enzyme mix (thermostable reverse transcriptase, hot-start Taq DNA polymerase and UDG enzyme) |
| Storage Conditions | Store at -25 to -15°C. |
| Shelf Life | 2 years when stored at -25 to -15°C. |
| Package Specifications | 100 T 1000 T 10000 T |
| Product Form | Liquid one-step RT-qPCR kit with separate buffer and enzyme mix components. |
| Expiration Date / Stability | Valid for 2 years when stored at -25 to -15°C. |
| Regulatory / Compliance | For research use and in vitro diagnostic reagent development. Not for direct clinical diagnosis. |
| Compatibility | Suitable for mainstream real-time PCR instruments and fast cycling programs. |
| Recommended Buffer System | Supplied optimized MP buffer containing Mg2+, dNTPs and the dUTP/UDG system. |
| Application Notes / Precautions | Handle RNA templates under RNase-free conditions. Include the UDG digestion step in the cycling program to ensure carryover control. |
For research use only, not for clinical use.
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