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| Product Name | Fast Cells-to-CT 2-Step RT-qPCR Probe Kit |
| Catalog No. | RESK-0079 |
| Description | A complete, fast and efficient kit that takes you directly from cells to amplification results, designed for quantitative gene expression analysis of 10 to 10^5 cultured cells (e.g., adherent and suspension cell lines, primary cultured cells, various stem cells and iPS cells). A unique lysis buffer system rapidly releases RNA from cultured cell samples, eliminating the time-consuming RNA purification process; the required RNA template is obtained in only 7 min. Together with the reverse transcription reagent (5x Direct RT Mix) and the probe-based qPCR reagent (2x TaqMan qPCR Mix) supplied in the kit, the entire workflow from template preparation to reverse transcription and gene expression analysis can be completed in as little as 1.5 h. The kit is suitable for high-throughput (96/384-well) analysis of cellular RNA expression. The 100 T format provides 96 lysis reactions, 96 reverse transcription reactions and 96 x 2 qPCR reactions, enough to process one full 96-well cell plate in a single run and avoid contamination and reagent performance loss from repeated opening and freeze-thaw. The 500 T format is intended for 384-well plates and higher-throughput automated screening platforms. |
| Intended Use | Quantitative gene expression analysis directly from cultured cells without RNA purification; suitable for high-throughput 96/384-well cellular RNA expression analysis. |
| Principle / Technology | Direct cell lysis rapidly releases RNA from cultured cells, followed by two-step RT-qPCR: separate reverse transcription of the lysate RNA into cDNA, then probe-based qPCR amplification and quantification. |
| Detection Method | Probe-based real-time quantitative PCR (fluorescent hydrolysis probe detection) |
| Sample Type | Cultured cells, 10 to 10^5 cells per reaction, including adherent and suspension cell lines, primary cultured cells, various stem cells and iPS cells |
| Performance Range / Specifications | Complete kit covering direct cell lysis, reverse transcription and qPCR amplification reagents RNA template ready in only 7 min; full workflow from template preparation to gene expression analysis in as little as 1.5 h Tolerates up to 30% cell lysate in the reaction Supports up to 384-well gene expression screening in a single run, more than 4x the throughput of conventional methods High precision with universal ROX reference dye included |
| Sensitivity / LOD | Quantitative analysis of gene expression from as few as 10 cultured cells |
| Reaction Conditions / Protocol | 1. Cell lysis: lyse cultured cells with the direct cell lysis reagent; RNA template is released within 7 min without RNA purification 2. Reverse transcription: reverse transcribe the lysate RNA using the 5x Direct RT Mix 3. qPCR: perform probe-based qPCR amplification with the 2x TaqMan qPCR Mix on a compatible real-time PCR instrument The complete workflow from cells to results can be finished in as little as 1.5 h |
| Components / Formulation | Direct cell lysis reagent 5x Direct RT Mix (reverse transcription reagent) 2x TaqMan qPCR Mix (probe-based qPCR reagent, with universal ROX) |
| Storage Conditions | Store Part I at 2 to 8 °C and Part II at -25 to -15 °C |
| Shelf Life | 1 year |
| Package Specifications | 100 T; 500 T |
| Product Form | Liquid reagent kit (multi-component) |
| Quality Control | High amplification precision and reproducibility, suitable for high-throughput screening |
| Expiration Date / Stability | Stable for 1 year when stored as recommended (Part I at 2 to 8 °C, Part II at -25 to -15 °C) |
| Regulatory / Compliance | For research use only |
| Compatibility | Contains universal ROX reference dye and is compatible with a wide range of real-time PCR instruments (high-ROX, low-ROX and no-ROX platforms) |
| Application Notes / Precautions | Process a full 96-well plate in a single run where possible to avoid contamination and reagent performance loss from repeated opening and freeze-thaw cycles The 500 T format is recommended for 384-well automated screening platforms No RNA purification step is required after lysis |
| Batch-to-Batch Consistency | High precision with low systematic error, suitable for high-throughput screening applications |
For research use only, not for clinical use.
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