- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | Fast Cells Lysis Kit V2 (for Direct RT-qPCR) |
| Catalog No. | RESK-0083 |
| Description | A rapid, high-efficiency kit for obtaining RNA from cultured cells, compatible with 10 to 10^5 cells per sample. Genomic DNA removal and RNA release are completed directly in the cell culture plate using a unique lysis buffer system that quickly liberates RNA from cultured cells, eliminating the time-consuming RNA purification step; the required RNA template is ready in just 7 minutes. The kit supports high-throughput (96/384-well) cellular RNA preparation for downstream applications such as reverse transcription and RT-qPCR amplification. The 100 T size covers a full 96-well plate in one run, avoiding contamination and performance loss from repeated reagent opening and freeze-thaw; the 500 T size suits 384-well and higher-throughput automated screening platforms. |
| Intended Use | Rapid lysis of cultured cells with simultaneous genomic DNA clearance to release RNA templates for downstream reverse transcription, RT-qPCR, and high-throughput gene expression analysis. |
| Principle / Technology | A specialized lysis buffer system disrupts cells directly in the culture plate and releases RNA while eliminating genomic DNA, producing amplification-ready RNA template without a purification step. |
| Sample Type | Cultured cells (10 to 10^5 cells per sample) in 96/384-well culture plates |
| Performance Range / Specifications | Compatible with 10 to 10^5 cultured cells per sample RNA template ready in 7 min; whole-plate RNA template preparation completed in 10 min Suitable for high-throughput automated screening platforms |
| Reaction Conditions / Protocol | Lysis and genomic DNA removal are performed directly in the cell culture plate; RNA template is obtained within approximately 7 min and can be used immediately in downstream reverse transcription or RT-qPCR. |
| Components / Formulation | Kit supplied as two parts (Part I and Part II), including the lysis buffer system for cell lysis, genomic DNA clearance, and RNA release |
| Storage Conditions | Part I: store at 2-8°C Part II: store at -25°C to -15°C |
| Shelf Life | 1 year |
| Package Specifications | 100 T; 500 T |
| Product Form | Liquid reagent kit |
| Shipping Conditions | Shipped on ice packs under controlled low temperature, keeping the kit below 4°C throughout transit |
| Expiration Date / Stability | Stable for 1 year when Part I is stored at 2-8°C and Part II at -25°C to -15°C |
| Compatibility | Compatible with downstream reverse transcription and RT-qPCR amplification workflows; suitable for 96-well and 384-well high-throughput automated screening platforms |
| Application Notes / Precautions | Process no more than 10^5 cells per sample within the validated range (10 to 10^5 cells) Use the 100 T size for single-run 96-well plate processing to avoid contamination and reagent degradation from repeated opening and freeze-thaw |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
