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| Product Name | Fast Cells Lysis Kit (for Direct RT-qPCR) |
| Catalog No. | RESK-0076 |
| Description | The Fast Cells Lysis Kit is a rapid, high-efficiency solution for obtaining RNA from cultured cells and is compatible with 10 to 100,000 cells per sample. Genomic DNA removal and RNA release are performed directly in the cell culture plate using a unique lysis buffer system that rapidly liberates RNA from cultured cell samples, eliminating the time-consuming RNA purification step. The kit supports high-throughput (96/384-well) cellular RNA preparation for downstream applications such as reverse transcription and RT-qPCR amplification. The 100 T format covers an entire 96-well cell plate in a single use, avoiding contamination and performance loss caused by repeated opening and freeze-thaw cycles, while the 500 T format suits 384-well and higher-throughput automated screening platforms. |
| Intended Use | Rapid preparation of RNA templates directly from cultured cells for reverse transcription, RT-qPCR, and high-throughput gene expression analysis. For research use only. |
| Principle / Technology | Direct chemical cell lysis: a proprietary lysis buffer releases RNA from cultured cells directly in the culture plate while eliminating genomic DNA, so no RNA purification is required. |
| Detection Method | Downstream reverse transcription and RT-qPCR amplification. |
| Sample Type | Cultured cells (adherent or suspension), 10 to 100,000 cells per sample. |
| Performance Range / Specifications | Fast and efficient: whole-plate RNA template preparation completed in 10 minutes. Suitable for high-throughput automated screening platforms (96/384-well formats). |
| Reaction Conditions / Protocol | Add lysis reagents directly to cultured cells in the culture plate; complete genomic DNA removal and RNA release in about 10 minutes; the resulting lysate can be used directly as template for reverse transcription and RT-qPCR. |
| Components / Formulation | Two-part kit (Part I and Part II) comprising a unique lysis buffer system for direct cell lysis, genomic DNA clearance, and RNA release. |
| Storage Conditions | Part I: store at 2-8°C. Part II: store at -25°C to -15°C. |
| Shelf Life | 1 year when stored as recommended. |
| Package Specifications | 100 T; 500 T |
| Shipping Conditions | Shipped with cold packs throughout transit to keep the kit below 4°C. |
| Expiration Date / Stability | Valid for 1 year under recommended storage conditions. |
| Compatibility | Compatible with 96-well and 384-well high-throughput automated screening platforms; the 100 T format supports one full 96-well cell plate in a single use, and the 500 T format suits 384-well and higher-throughput workflows. |
| Application Notes / Precautions | Use the 100 T format for one complete 96-well plate in a single session to avoid contamination and reagent performance degradation caused by repeated opening and freeze-thaw cycles. |
For research use only, not for clinical use.
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