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| Product Name | C55P1 TaqMan Multiplex qPCR Master Mix (UDG Plus) |
| Catalog No. | RESK-0098 |
| Description | The C55P1 TaqMan Multiplex qPCR Master Mix (UDG Plus) is a full premix real-time quantitative PCR reagent based on a next-generation antibody-based hot-start Taq DNA polymerase. The 2× mix contains both the reaction buffer and the enzyme blend, including Mg2+ and dNTPs, plus factors that suppress non-specific amplification and enhance multiplex amplification efficiency, enabling up to 4-plex reactions while maintaining primer efficiency. The product contains UDG and uses a dUTP/UDG carryover prevention system that effectively eliminates the risk of aerosol contamination from previous PCR products. |
| Intended Use | Development of multiplex real-time qPCR assays, including full-premix formulations with primers and probes pre-added, for molecular diagnostic reagent development and research. |
| Principle / Technology | Probe-based (TaqMan) real-time quantitative PCR using a next-generation antibody-modified hot-start Taq DNA polymerase. Polymerase activity is blocked at ambient temperature and activated by heat, minimizing non-specific amplification and primer-dimer formation. The incorporated dUTP/UDG anti-contamination system degrades uracil-bearing carryover amplicons before amplification, preventing aerosol-borne product contamination and reducing false positives. |
| Detection Method | TaqMan probe-based real-time fluorescence quantitative PCR (qPCR). |
| Sample Type | DNA templates. |
| Performance Range / Specifications | Supports up to 4-plex qPCR reactions. Supports advance pre-mixing of primers and probes into a full premix with strong stability. Stable detection of low-concentration templates (down to Ct 37). dUTP/UDG anti-contamination system effectively prevents aerosol contamination. |
| Sensitivity / LOD | Stable detection of low-concentration templates at Ct 37. |
| Specificity | The buffer contains factors that effectively suppress non-specific PCR amplification and factors that enhance amplification efficiency in multiplex qPCR, ensuring primer efficiency in up to 4-plex reactions. |
| Reaction Conditions / Protocol | 2× full premix containing both buffer and enzymes; add primers, probes and template to set up the reaction. Compatible with primers/probes pre-mixed in advance. |
| Components / Formulation | 2× master mix containing reaction buffer, antibody-based hot-start Taq DNA polymerase, Mg2+, dNTPs (with dUTP) and UDG enzyme. |
| Storage Conditions | Store at -25 to -15°C. |
| Shelf Life | 2 years when stored at -25 to -15°C. |
| Package Specifications | 1 mL 5 mL 5× 1 mL 100 mL |
| Product Form | 2× full premix (buffer and enzymes combined in a single solution). |
| Expiration Date / Stability | Valid for 2 years when stored at -25 to -15°C. Full premix with primers and probes shows no significant change in Ct values or fluorescence after 7 days at 37°C. Stable after 15 freeze-thaw cycles (-20°C storage with slow thawing at room temperature), with no significant change in Ct values or fluorescence versus control. |
| Regulatory / Compliance | For research use and in vitro diagnostic reagent development. Not for direct clinical diagnosis. |
| Compatibility | Compatible with multiple real-time PCR platforms and reaction systems, across mainstream platforms. |
| Recommended Buffer System | Ready-to-use 2× full premix containing Mg2+ and dNTPs; no additional buffer required. |
| Application Notes / Precautions | The dUTP/UDG system requires an appropriate UDG digestion step in the cycling program; avoid amplicons lacking dUTP when carryover control is needed. |
For research use only, not for clinical use.
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