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| Product Name | C15P2 TaqMan Multiplex qPCR Probe Kit |
| Catalog No. | RESK-0097 |
| Description | The C15P2 TaqMan Multiplex qPCR Probe Kit is a real-time quantitative PCR reagent built on a next-generation antibody-based hot-start Taq DNA polymerase. The 2× reaction mix already contains Mg2+ and dNTPs and is supplemented with factors that effectively suppress non-specific PCR amplification and factors that improve amplification efficiency in multiplex qPCR, allowing up to 4-plex reactions while maintaining primer efficiency. The kit is supplied in a convenient two-component format for easy assay optimization. |
| Intended Use | Development of multiplex real-time qPCR assays for molecular diagnostic reagent development and research applications. |
| Principle / Technology | Probe-based (TaqMan) real-time quantitative PCR using a next-generation antibody-modified hot-start Taq DNA polymerase. Polymerase activity is blocked at ambient temperature and activated by heat, minimizing non-specific amplification and primer-dimer formation. |
| Detection Method | TaqMan probe-based real-time fluorescence quantitative PCR (qPCR). |
| Sample Type | DNA templates. |
| Performance Range / Specifications | Supports up to 4-plex qPCR reactions while maintaining primer amplification efficiency. |
| Specificity | The buffer contains factors that effectively suppress non-specific PCR amplification and factors that enhance amplification efficiency in multiplex qPCR, ensuring primer efficiency in up to 4-plex reactions. |
| Reaction Conditions / Protocol | Two-component format: combine the 2× master mix with primers, probes and template according to the target assay; the format facilitates flexible optimization of the reaction system. |
| Components / Formulation | 2× reaction mix (contains reaction buffer, Mg2+ and dNTPs) Antibody-based hot-start Taq DNA polymerase |
| Storage Conditions | Store at -25 to -15°C. |
| Shelf Life | 2 years when stored at -25 to -15°C. |
| Package Specifications | 100 T 1000 T 10000 T |
| Product Form | Two-component liquid format (2× master mix and enzyme), convenient for reaction system optimization. |
| Expiration Date / Stability | Valid for 2 years when stored at -25 to -15°C. |
| Regulatory / Compliance | For research use and in vitro diagnostic reagent development. Not for direct clinical diagnosis. |
| Compatibility | Suitable for mainstream real-time PCR instruments. |
| Recommended Buffer System | Ready-to-use 2× master mix containing Mg2+ and dNTPs; no additional buffer required. |
For research use only, not for clinical use.
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