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| Product Name | Advanced Fast One-Step RT-qPCR Probe Mix V2 |
| Catalog No. | RESK-0082 |
| Description | A one-step, probe-based RT-qPCR reagent designed for rapid reverse transcription and amplification of RNA templates released directly from cultured cell lysates. Reverse transcription and qPCR amplification are performed in a single tube, shortening total reaction time and reducing the risk of contamination. The formulation offers strong tolerance to lysate carryover and broad instrument compatibility. It is well suited for high-throughput (96/384-well) cellular RNA expression analysis: the 100 T size provides enough reagent for two full 96-well plates, avoiding repeated opening and freeze-thaw of reagents, while the 500 T size supports 384-well automated screening platforms. |
| Intended Use | One-step probe-based reverse transcription and quantitative PCR amplification of RNA directly from cultured cell lysates, for gene expression analysis in standard and high-throughput (96/384-well) formats. |
| Principle / Technology | One-step RT-qPCR: reverse transcriptase synthesizes cDNA and DNA polymerase amplifies the target in the same closed tube; sequence-specific hydrolysis probes generate a fluorescence signal proportional to target abundance. |
| Detection Method | Probe-based real-time quantitative PCR (fluorescence detection) |
| Sample Type | Cell lysates prepared from cultured cells (direct RT-qPCR without RNA purification) |
| Performance Range / Specifications | Tolerates up to 30% cell lysate in the reaction Supports one-time screening of up to 384 wells, a >4-fold throughput improvement over conventional methods High precision; contains a universal ROX component for compatibility with various qPCR instruments |
| Specificity | Sequence-specific detection via target-specific primers and hydrolysis probes |
| Reaction Conditions / Protocol | Reverse transcription and qPCR amplification are carried out sequentially in a single closed-tube reaction; no intermediate handling step is required. |
| Components / Formulation | One-step RT-qPCR Mix (contains reverse transcriptase, DNA polymerase, reaction buffer, dNTPs, and a universal ROX reference component) |
| Storage Conditions | Store at -25°C to -15°C. |
| Shelf Life | 1 year |
| Package Specifications | 100 T; 500 T |
| Product Form | Liquid master mix |
| Expiration Date / Stability | Stable for 1 year when stored at -25°C to -15°C; the 100 T format is sized for single-plate use to minimize performance loss from repeated opening and freeze-thaw cycles |
| Compatibility | Compatible with a wide range of real-time PCR instruments (universal ROX included); suitable for 96-well and 384-well high-throughput and automated screening platforms |
| Application Notes / Precautions | Use with cell lysates prepared by a compatible direct cell lysis procedure To avoid contamination and reagent degradation, use the 100 T size for single-run 96-well plate processing Validated for lysate carryover up to 30% of the reaction volume |
For research use only, not for clinical use.
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